CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004335
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytochrome P450 2A4 
Protein Synonyms/Alias
 CYPIIA4; Cytochrome P450-15-alpha; Cytochrome P450-IIA3.1; Testosterone 15-alpha-hydroxylase 
Gene Name
 Cyp2a4 
Gene Synonyms/Alias
 Cyp2a-4 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
142LRDFGVGKRGIEERIubiquitination[1]
162FLIDSFRKTNGAFIDubiquitination[1]
239GPQQQAFKELQGLEDubiquitination[1]
250GLEDFITKKVEHNQRubiquitination[1]
251LEDFITKKVEHNQRTubiquitination[1]
280IRMLEEKKNPNTEFYubiquitination[1]
420PKHFLDDKGQFKKSDubiquitination[1]
425DDKGQFKKSDAFVPFubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Highly active in the 15-alpha-hydroxylation of testosterone. Also active in the 15-alpha-hydroxylation of progesterone and androstenedione. Little or no activity on corticosterone, pregnenolone, dehydroepiandrosterone, estradiol or estriol. 
Sequence Annotation
 METAL 439 439 Iron (heme axial ligand).  
Keyword
 Complete proteome; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 494 AA 
Protein Sequence
MLTSGLLLVA AVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFVGNFLQLN TEQMYNSLMK 60
ISQRYGPVFT IYLGSRRIVV LCGQETVKEA LVDQAEEFSG RGEQATFDWL FKGYGIAFSS 120
GERAKQLRRF SITTLRDFGV GKRGIEERIQ EEAGFLIDSF RKTNGAFIDP TFYLSRTVSN 180
VISSIVFGDR FDYEDKEFLS LLRMMLGSLQ FTATSMGQVY EMFSSVMKHL PGPQQQAFKE 240
LQGLEDFITK KVEHNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF 300
AGTETVSTTL RYGFLLLMKY PDIEAKVHEE IDRVIGRNRQ PKYEDRMKMP YTEAVIHEIQ 360
RFADLIPMGL ARRVTKDTKF RDFLLPKGTE VFPMLGSVLK DPKFFSNPKD FNPKHFLDDK 420
GQFKKSDAFV PFSIGKRYCF GEGLARMELF LFLTNIMQNF HFKSTQEPQD IDVSPRLVGF 480
VTIPPTYTMS FLSR 494 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0070330; F:aromatase activity; IEA:EC.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0035634; P:response to stilbenoid; IEP:UniProtKB. 
Interpro
 IPR001128; Cyt_P450.
 IPR017972; Cyt_P450_CS.
 IPR002401; Cyt_P450_E_grp-I.
 IPR008067; Cyt_P450_E_grp-I_CYP2A-like. 
Pfam
 PF00067; p450 
SMART
  
PROSITE
 PS00086; CYTOCHROME_P450 
PRINTS
 PR00463; EP450I.
 PR01684; EP450ICYP2A.
 PR00385; P450.