CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012963
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ral guanine nucleotide dissociation stimulator-like 3 
Protein Synonyms/Alias
 RalGDS-like 3 
Gene Name
 RGL3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
129PPGVEIKKTAVQDLSubiquitination[1]
139VQDLSFNKNLRAVVSubiquitination[1, 2]
187PGSAEAQKAEKLLEDubiquitination[1, 2]
375EPLSTFRKLSQIFSDubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Guanine nucleotide exchange factor (GEF) for Ral-A. Potential effector of GTPase HRas and Ras-related protein M-Ras. Negatively regulates Elk-1-dependent gene induction downstream of HRas and MEKK1 (By similarity). 
Sequence Annotation
 DOMAIN 65 200 N-terminal Ras-GEF.
 DOMAIN 247 505 Ras-GEF.
 DOMAIN 613 700 Ras-associating.
 REGION 612 707 Interaction with HRAS, MRAS and RIT1 (By
 MOD_RES 52 52 Phosphoserine.
 MOD_RES 508 508 Phosphoserine (By similarity).
 MOD_RES 512 512 Phosphoserine.
 MOD_RES 569 569 Phosphoserine (By similarity).
 MOD_RES 573 573 Phosphoserine (By similarity).
 MOD_RES 591 591 Phosphoserine.
 MOD_RES 601 601 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Guanine-nucleotide releasing factor; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 710 AA 
Protein Sequence
MERTAGKELA LAPLQDWGEE TEDGAVYSVS LRRQRSQRRS PAEGPGGSQA PSPIANTFLH 60
YRTSKVRVLR AARLERLVGE LVFGDREQDP SFMPAFLATY RTFVPTACLL GFLLPPMPPP 120
PPPGVEIKKT AVQDLSFNKN LRAVVSVLGS WLQDHPQDFR DPPAHSDLGS VRTFLGWAAP 180
GSAEAQKAEK LLEDFLEEAE REQEEEPPQV WTGPPRVAQT SDPDSSEACA EEEEGLMPQG 240
PQLLDFSVDE VAEQLTLIDL ELFSKVRLYE CLGSVWSQRD RPGAAGASPT VRATVAQFNT 300
VTGCVLGSVL GAPGLAAPQR AQRLEKWIRI AQRCRELRNF SSLRAILSAL QSNPIYRLKR 360
SWGAVSREPL STFRKLSQIF SDENNHLSSR EILFQEEATE GSQEEDNTPG SLPSKPPPGP 420
VPYLGTFLTD LVMLDTALPD MLEGDLINFE KRRKEWEILA RIQQLQRRCQ SYTLSPHPPI 480
LAALHAQNQL TEEQSYRLSR VIEPPAASCP SSPRIRRRIS LTKRLSAKLA REKSSSPSGS 540
PGDPSSPTSS VSPGSPPSSP RSRDAPAGSP PASPGPQGPS TKLPLSLDLP SPRPFALPLG 600
SPRIPLPAQQ SSEARVIRVS IDNDHGNLYR SILLTSQDKA PSVVRRALQK HNVPQPWACD 660
YQLFQVLPGD RVLLIPDNAN VFYAMSPVAP RDFMLRRKEG TRNTLSVSPS 710 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0008321; F:Ral guanyl-nucleotide exchange factor activity; IEA:Compara.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR000159; Ras-assoc.
 IPR000651; Ras-like_Gua-exchang_fac_N.
 IPR019804; Ras_G-nucl-exch_fac_CS.
 IPR008937; Ras_GEF.
 IPR023578; Ras_GEF_dom.
 IPR001895; RasGRF_CDC25. 
Pfam
 PF00788; RA
 PF00617; RasGEF
 PF00618; RasGEF_N 
SMART
 SM00314; RA
 SM00147; RasGEF
 SM00229; RasGEFN 
PROSITE
 PS50200; RA
 PS00720; RASGEF
 PS50009; RASGEF_CAT
 PS50212; RASGEF_NTER 
PRINTS