UniProt Accession

 ERF1_YEAST; P12385; D6VQD9 

Genbank Protein ID

 X04082; M28042; Z36012; X73531; BK006936 

Genbank Nucleotide ID

 CAA27719.1; CAA85101.1; CAA51935.1; DAA07259.1 

Protein Name

 Eukaryotic peptide chain release factor subunit 1 

Protein Synonyms/Alias

 Eukaryotic release factor 1; eRF1; Omnipotent suppressor protein 1 

Gene Name

 SUP45 

Gene Synonyms/Alias

 SAL4; SUP1; YBR143C; YBR1120 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
23	KLVQSLEKARGNGTS	acetylation	[1]
47	GQIPLYQKMLTDEYG	ubiquitination	[2]
60	YGTASNIKSRVNRLS	ubiquitination	[2]
168	NTRTVLHKFTVDLPK	acetylation	[1]
215	QNFITNDKVNVKGLI	acetylation	[1]
235	DFKTDLAKSELFDPR	acetylation	[1]
331	ETIRYTFKDAEDNEV	ubiquitination	[2, 3, 4]
347	KFAEPEAKDKSFAID	ubiquitination	[2]
349	AEPEAKDKSFAIDKA	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.
 Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH, Kleifeld O.
 Mol Cell Proteomics. 2011 May;10(5):M111.009753. [PMID: 21427232]
 [4] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA (By similarity). 

Sequence Annotation

 MOD_RES 182 182 N5-methylglutamine.
 MOD_RES 421 421 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Methylation; Phosphoprotein; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 437 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IPI:SGD.
 GO:0018444; C:translation release factor complex; IDA:SGD.
 GO:0016149; F:translation release factor activity, codon specific; IMP:SGD.
 GO:0000910; P:cytokinesis; IMP:SGD. 

Interpro

 IPR005140; eRF1_1_Pelota.
 IPR005141; eRF1_2.
 IPR005142; eRF1_3.
 IPR004403; Peptide_chain-rel_eRF1/aRF1.
 IPR024049; Release_factor_eRF1/aRF1_N. 

Pfam

 PF03463; eRF1_1
 PF03464; eRF1_2
 PF03465; eRF1_3 

SMART

  

PROSITE

  

PRINTS