CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031358
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 cDNA FLJ55484, highly similar to ATP-dependent RNA helicase DDX39 (EC 3.6.1.-) 
Protein Synonyms/Alias
  
Gene Name
  
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
78APPKKDIKGSYVSIHubiquitination[1, 2]
95GFRDFLLKPELLRAIubiquitination[1, 2, 3]
173ELAFQISKEYERFSKubiquitination[1]
180KEYERFSKYMPSVKVubiquitination[1, 3]
204KKDEEVLKKNCPHVVubiquitination[1]
205KDEEVLKKNCPHVVVubiquitination[1, 4]
230RNRSFSLKNVKHFVLubiquitination[1]
264FRLTPHEKQCMMFSAubiquitination[1]
283DIRPVCRKFMQDPMEubiquitination[1]
310GLQQYYVKLKDSEKNubiquitination[1, 2]
376LSRYQQFKDFQRRILubiquitination[2, 3]
426RAGRFGTKGLAITFVubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Helicase; Hydrolase; Nucleotide-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 470 AA 
Protein Sequence
MAYGAPSPTW SLSVRPNRPR GGLGPFGEVQ VILLPPSSWD YSIMAEQDVE NDLLDYDEEE 60
EPQAPQESTP APPKKDIKGS YVSIHSSGFR DFLLKPELLR AIVDCGFEHP SEVQHECIPQ 120
AILGMDVLCQ AKSGMGKTAV FVLATLQQIE PVNGQVTVLV MCHTRELAFQ ISKEYERFSK 180
YMPSVKVSVF FGGLSIKKDE EVLKKNCPHV VVGTPGRILA LVRNRSFSLK NVKHFVLDEC 240
DKMLGQLDMR RDVQEIFRLT PHEKQCMMFS ATLSKDIRPV CRKFMQDPME VFVDDETKLT 300
LHGLQQYYVK LKDSEKNRKL FDLLDVLEFN QVIIFVKSVQ RCMALAQLLV EQNFPAIAIH 360
RGMAQEERLS RYQQFKDFQR RILVATNLFG RGMDIERVNI VFNYDMPEDS DTYLHRVARA 420
GRFGTKGLAI TFVSDENDAK ILNDVQDRFE VNVAELPEEI DISTYIEQSR 470 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS