UniProt Accession

 MAZF_ECOLI; P0AE70; P33645; Q2MA50 

Genbank Protein ID

 D16450; J04039; U29580; U00096; AP009048 

Genbank Nucleotide ID

 BAA03918.1; AAA03239.1; AAA69292.1; AAC75824.1; BAE76856.1 

Protein Name

 mRNA interferase MazF 

Protein Synonyms/Alias

 Endoribonuclease MazF; Toxin MazF 

Gene Name

 mazF 

Gene Synonyms/Alias

 chpA; chpAK; b2782; JW2753 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
105	ELQLIKAKINVLIG*	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Toxic component of a toxin-antitoxin (TA) module. MazF is a sequence-specific mRNA endoribonuclease that inhibits protein synthesis and induces bacterial stasis. It is very stable, single- strand specific and cleavage is independent of the ribosome, although translation enhances cleavage for some mRNAs. Cleavage usually occurs between the first A and C of ACA sequences, yielding a 2',3'-cyclic phosphate and a free 5'-OH. The endoribonuclease activity (a toxin) is inhibited by the labile cognate antitoxin MazE. Toxicity results when the levels of MazE decrease in the cell, leading to mRNA degradation. This effect can be rescued by expression of MazE, but after 6 hours in rich medium overexpression of MazF leads to programmed cell death. MazF- mediated cell death occurs following a number of stress conditions in a relA-dependent fashion and only when cells are in log phase. Cell growth and viability are not affected when MazF and MazE are coexpressed. Programmed cell death occurs when cells are at high density and depends on the presence of MazEF and a quorum sensing pentapeptide, the extracellular death factor (EDF) with sequence NNWNN, probably produced from the zwg gene product glucose-6- phosphate 1-dehydrogenase. Both MazE and MazEF bind to the promoter region of the mazEF operon to inhibit their transcription. 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; DNA-binding; Endonuclease; Hydrolase; Nuclease; Quorum sensing; Reference proteome; Repressor; RNA-binding; Stress response; Toxin; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 111 AA 

Protein Sequence

Gene Ontology

 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0004521; F:endoribonuclease activity; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0030308; P:negative regulation of cell growth; IMP:UniProtKB.
 GO:0009372; P:quorum sensing; IEA:UniProtKB-KW.
 GO:0043488; P:regulation of mRNA stability; IDA:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR003477; PemK.
 IPR011067; Plasmid_toxin/cell-grow_inhib. 

Pfam

 PF02452; PemK 

SMART

  

PROSITE

  

PRINTS