CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031226
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 26S proteasome non-ATPase regulatory subunit 3 
Protein Synonyms/Alias
 cDNA FLJ54148, highly similar to 26S proteasome non-ATPase regulatory subunit 3 
Gene Name
 PSMD3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
56ISDDLMQKISTQNRRubiquitination[1, 2]
71ALDLVAAKCYYYHARubiquitination[2, 3]
85RVYEFLDKLDVVRSFubiquitination[1, 3, 4]
131SLYDQAEKLVSKSVFubiquitination[4, 5]
135QAEKLVSKSVFPEQAubiquitination[1, 2, 3, 4, 5, 6]
158LYYTGRIKAIQLEYSubiquitination[1, 2, 3, 5, 6]
236VRTGNLAKFNQVLDQubiquitination[1, 4, 5, 6]
266RLRHNVIKTGVRMISubiquitination[3]
287SLADIAQKLQLDSPEubiquitination[1, 5, 6]
302DAEFIVAKAIRDGVIubiquitination[1, 4, 5, 6, 7]
317EASINHEKGYVQSKEacetylation[8]
317EASINHEKGYVQSKEubiquitination[1, 5]
323EKGYVQSKEMIDIYSubiquitination[1, 2, 4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Proteasome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 356 AA 
Protein Sequence
MDTEADLQFR PRTGKAASTP LLPEVEAYLQ LLVVIFMMNS KRYKEAQKIS DDLMQKISTQ 60
NRRALDLVAA KCYYYHARVY EFLDKLDVVR SFLHARLRTA TLRHDADGQA TLLNLLLRNY 120
LHYSLYDQAE KLVSKSVFPE QANNNEWARY LYYTGRIKAI QLEYSEARRT MTNALRKAPQ 180
HTAVGFKQTV HKLLIVVELL LGEIPDRLQF RQPSLKRSLM PYFLLTQAVR TGNLAKFNQV 240
LDQFGEKFQA DGTYTLIIRL RHNVIKTGVR MISLSYSRIS LADIAQKLQL DSPEDAEFIV 300
AKAIRDGVIE ASINHEKGYV QSKEMIDIYS TREPQLAFHQ RISFCLDIHN MSVKGW 356 
Gene Ontology
 GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
 GO:0030234; F:enzyme regulator activity; IEA:InterPro.
 GO:0050790; P:regulation of catalytic activity; IEA:GOC.
 GO:0042176; P:regulation of protein catabolic process; IEA:InterPro. 
Interpro
 IPR013586; 26S_Psome_reg_C.
 IPR013143; PAM.
 IPR000717; PCI_dom.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF01399; PCI
 PF08375; Rpn3_C 
SMART
 SM00753; PAM
 SM00088; PINT 
PROSITE
  
PRINTS