CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018359
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcriptional repressor p66-beta 
Protein Synonyms/Alias
 GATA zinc finger domain-containing protein 2B; p66/p68 
Gene Name
 GATAD2B 
Gene Synonyms/Alias
 KIAA1150 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
30ERDDVLAKRLKMEGHubiquitination[1, 2]
33DVLAKRLKMEGHEAMsumoylation[3]
44HEAMERLKMLALLKRubiquitination[4]
78GVKGYEEKLNGNLRPacetylation[5]
97RTAGRPGKENINDEPubiquitination[1]
451CMTSNQKKALKAEHTubiquitination[1]
467RLKNAFVKALQQEQEubiquitination[1, 2, 4]
498PAVSSVSKQETIMRHubiquitination[1]
593SQSISGQK*******ubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] SUMO modification enhances p66-mediated transcriptional repression of the Mi-2/NuRD complex.
 Gong Z, Brackertz M, Renkawitz R.
 Mol Cell Biol. 2006 Jun;26(12):4519-28. [PMID: 16738318]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2A. Targets MBD3 to discrete loci in the nucleus. May play a role in synapse development. 
Sequence Annotation
 ZN_FING 414 467 GATA-type.
 REGION 165 195 CR1; MBD2- and MBD3-binding.
 REGION 340 480 CR2; histone tail-binding.
 MOD_RES 120 120 Phosphothreonine.
 MOD_RES 122 122 Phosphoserine.
 MOD_RES 129 129 Phosphoserine.
 MOD_RES 134 134 Phosphoserine.
 MOD_RES 135 135 Phosphoserine.
 MOD_RES 333 333 Phosphoserine.
 MOD_RES 338 338 Phosphoserine.
 MOD_RES 486 486 Phosphoserine.  
Keyword
 Coiled coil; Complete proteome; Mental retardation; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 593 AA 
Protein Sequence
MDRMTEDALR LNLLKRSLDP ADERDDVLAK RLKMEGHEAM ERLKMLALLK RKDLANLEVP 60
HELPTKQDGS GVKGYEEKLN GNLRPHGDNR TAGRPGKENI NDEPVDMSAR RSEPERGRLT 120
PSPDIIVLSD NEASSPRSSS RMEERLKAAN LEMFKGKGIE ERQQLIKQLR DELRLEEARL 180
VLLKKLRQSQ LQKENVVQKT PVVQNAASIV QPSPAHVGQQ GLSKLPSRPG AQGVEPQNLR 240
TLQGHSVIRS ATNTTLPHML MSQRVIAPNP AQLQGQRGPP KPGLVRTTTP NMNPAINYQP 300
QSSSSVPCQR TTSSAIYMNL ASHIQPGTVN RVSSPLPSPS AMTDAANSQA AAKLALRKQL 360
EKTLLEIPPP KPPAPLLHFL PSAANSEFIY MVGLEEVVQS VIDSQGKSCA SLLRVEPFVC 420
AQCRTDFTPH WKQEKNGKIL CEQCMTSNQK KALKAEHTNR LKNAFVKALQ QEQEIEQRLQ 480
QQAALSPTTA PAVSSVSKQE TIMRHHTLRQ APQPQSSLQR GIPTSARSML SNFAQAPQLS 540
VPGGLLGMPG VNIAYLNTGI GGHKGPSLAD RQREYLLDMI PPRSISQSIS GQK 593 
Gene Ontology
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR000679; Znf_GATA.
 IPR013088; Znf_NHR/GATA. 
Pfam
 PF00320; GATA 
SMART
  
PROSITE
 PS00344; GATA_ZN_FINGER_1
 PS50114; GATA_ZN_FINGER_2 
PRINTS