| Tag | Content |
|---|
| CPLM ID | CPLM-007749 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | ATP-dependent RNA helicase ROK1 |
Protein Synonyms/Alias | Rescuer of KEM1 protein 1 |
Gene Name | ROK1 |
Gene Synonyms/Alias | YGL171W; G1651 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 60 | RNKRIISKVEDDREK | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA. May also have a gene-specific regulatory function since it affects nuclear fusion by regulating KAR4 expression and contributes with KEM1 to ISP-1 sensitivity. |
Sequence Annotation | DOMAIN 153 333 Helicase ATP-binding.
DOMAIN 344 506 Helicase C-terminal.
NP_BIND 166 173 ATP (By similarity).
MOTIF 122 150 Q motif.
MOTIF 280 283 DEAD box. |
Keyword | ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 564 AA |
Protein Sequence | MDIFRVLTRG ASVKKESGPK AKAADYSVIN GNDENHKEDN NESQIVKELD FFRNKRIISK 60
VEDDREKTTE NDSPNKEEKS GNDDGLIKPV ITNTVEASAL RKSYKGNVSG IDIPLPIGSF 120
EDLISRFSFD KRLLNNLIEN GFTEPTPIQC ECIPVALNNR DVLACGPTGS GKTLAFLIPL 180
VQQIIDDKQT AGLKGLIISP TKELANQIFI ECFKLSYKIF LEKKRPLQVA LLSKSLGAKL 240
KNKVVSDKKY DIIISTPLRL IDVVKNEALD LSKVKHLIFD EADKLFDKTF VEQSDDILSA 300
CREPSLRKAM FSATIPSNVE EIAQSIMMDP VRVIIGHKEA ANTNIEQKLI FCGNEEGKLI 360
AIRQLVQEGE FKPPIIIFLE SITRAKALYH ELMYDRINVD VIHAERTALQ RDRIIERFKT 420
GELWCLICTD VLARGIDFKG VNLVINYDVP GSSQAYVHRI GRTGRGGRSG KAITFYTKQD 480
SVAIKPIINV MKQSGCEVSE WMDKMAKMTR KEKESIKNGK AHKERKQITT VPKMDKAKRR 540
RQQEMIAASK RRKNEELSKK HFSK 564 |
Gene Ontology | GO:0030686; C:90S preribosome; IDA:SGD. GO:0005730; C:nucleolus; IDA:SGD. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0004004; F:ATP-dependent RNA helicase activity; IDA:SGD. GO:0003723; F:RNA binding; IEA:UniProtKB-KW. GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD. GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD. GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD. GO:0048254; P:snoRNA localization; IMP:SGD. |
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