CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017162
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Parafibromin 
Protein Synonyms/Alias
 Cell division cycle protein 73 homolog; Hyperparathyroidism 2 protein homolog 
Gene Name
 Cdc73 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
209DDDITALKQRSFVDAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Tumor suppressor probably involved in transcriptional and post-transcriptional control pathways. May be involved in cell cycle progression through the regulation of cyclin D1/PRAD1 expression. Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys- 4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Connects PAF1C with the cleavage and polyadenylation specificity factor (CPSF) complex and the cleavage stimulation factor (CSTF) complex, and with Wnt signaling. Involved in polyadenylation of mRNA precursors (By similarity). 
Sequence Annotation
 REGION 200 531 Interaction with POLR2A and PAF1 (By
 REGION 200 250 Interaction with CTNNB1 (By similarity).
 MOTIF 125 139 Nuclear localization signal (By
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 212 212 Phosphoserine (By similarity).  
Keyword
 Acetylation; Cell cycle; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Tumor suppressor; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 531 AA 
Protein Sequence
MADVLSVLRQ YNIQKKEIVV KGDEVIFGEF SWPKNVKTNY VVWGTGKEGQ PREYYTLDSI 60
LFLLNNVHLS HPVYVRRAAT ENIPVVRRPD RKDLLGYLNG EASTSASIDR SAPLEIGLQR 120
STQVKRAADE VLAEAKKPRI EDEECVRLDK ERLAARLEGH KEGIVQTEQI RSLSEAMSVE 180
KIAAIKAKIM AKKRSTIKTD LDDDITALKQ RSFVDAEVDV TRDIVSRERV WRTRTTILQS 240
TGKNFSKNIF AILQSVKARE EGRAPEQRPA PNAAPVDPTL RTKQPIPAAY NRYDQERFKG 300
KEETEGFKID TMGTYHGMTL KSVTEGASAR KTQTPAAQPV PRPVSQARPP PNQKKGSRTP 360
IIIIPAATTS LITMLNAKDL LQDLKFVPSD EKKKQGCQRE NETLIQRRKD QMQPGGTAIS 420
VTVPYRVVDQ PLKLMPQDWD RVVAVFVQGP AWQFKGWPWL LPDGSPVDIF AKIKAFHLKY 480
DEVRLDPNVQ KWDVTVLELS YHKRHLDRPV FLRFWETLDR YMVKHKSHLR F 531 
Gene Ontology
 GO:0016593; C:Cdc73/Paf1 complex; ISS:UniProtKB.
 GO:0000993; F:RNA polymerase II core binding; ISS:UniProtKB.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0071222; P:cellular response to lipopolysaccharide; IDA:UniProtKB.
 GO:0001711; P:endodermal cell fate commitment; IMP:UniProtKB.
 GO:0033523; P:histone H2B ubiquitination; IEA:Compara.
 GO:0010390; P:histone monoubiquitination; IEA:Compara.
 GO:0006378; P:mRNA polyadenylation; ISS:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; ISS:UniProtKB.
 GO:0050680; P:negative regulation of epithelial cell proliferation; IEA:Compara.
 GO:0048147; P:negative regulation of fibroblast proliferation; IEA:Compara.
 GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; ISS:UniProtKB.
 GO:0045638; P:negative regulation of myeloid cell differentiation; ISS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
 GO:0031442; P:positive regulation of mRNA 3'-end processing; ISS:UniProtKB.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0030177; P:positive regulation of Wnt receptor signaling pathway; ISS:UniProtKB.
 GO:0031648; P:protein destabilization; IEA:Compara.
 GO:0019827; P:stem cell maintenance; IMP:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR007852; RNA_pol_access_fac_Cdc73. 
Pfam
 PF05179; CDC73 
SMART
  
PROSITE
  
PRINTS