CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013316
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Anoctamin-6 
Protein Synonyms/Alias
 Small-conductance calcium-activated nonselective cation channel; SCAN channel; Transmembrane protein 16F 
Gene Name
 ANO6 
Gene Synonyms/Alias
 TMEM16F 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
56EFEEFNGKPDSLFFNubiquitination[1, 2, 3, 4]
120TRSVLDDKLVFVKVHubiquitination[2]
176SVDESIIKPEQEFFTubiquitination[1, 4]
188FFTAPFEKNRMNDFYubiquitination[1]
229QVINNVSKFGINRLVubiquitination[2]
281HPRSIYKKQPLDLIRubiquitination[3]
589DPVYWLGKYRNEECDubiquitination[1, 5]
644HRVSGSEKITPRWEQubiquitination[1, 3]
793ADFKNKSKGNPYSDLubiquitination[3]
876REKYLTQKLLHENHLubiquitination[6]
888NHLKDMTKNMGVIAEubiquitination[1, 2, 3, 4, 6, 7]
908VDNNLRPKSE*****ubiquitination[1, 3, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets and osteoblasts. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes. 
Sequence Annotation
 CARBOHYD 329 329 N-linked (GlcNAc...) (Potential).
 CARBOHYD 361 361 N-linked (GlcNAc...) (Potential).
 CARBOHYD 493 493 N-linked (GlcNAc...).
 CARBOHYD 777 777 N-linked (GlcNAc...) (Potential).
 CARBOHYD 790 790 N-linked (GlcNAc...) (Potential).
 CARBOHYD 802 802 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Cell membrane; Chloride; Chloride channel; Complete proteome; Glycoprotein; Ion channel; Ion transport; Membrane; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Transport; Voltage-gated channel. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 910 AA 
Protein Sequence
MKKMSRNVLL QMEEEEDDDD GDIVLENLGQ TIVPDLGSLE SQHDFRTPEF EEFNGKPDSL 60
FFNDGQRRID FVLVYEDESR KETNKKGTNE KQRRKRQAYE SNLICHGLQL EATRSVLDDK 120
LVFVKVHAPW EVLCTYAEIM HIKLPLKPND LKNRSSAFGT LNWFTKVLSV DESIIKPEQE 180
FFTAPFEKNR MNDFYIVDRD AFFNPATRSR IVYFILSRVK YQVINNVSKF GINRLVNSGI 240
YKAAFPLHDC KFRRQSEDPS CPNERYLLYR EWAHPRSIYK KQPLDLIRKY YGEKIGIYFA 300
WLGYYTQMLL LAAVVGVACF LYGYLNQDNC TWSKEVCHPD IGGKIIMCPQ CDRLCPFWKL 360
NITCESSKKL CIFDSFGTLV FAVFMGVWVT LFLEFWKRRQ AELEYEWDTV ELQQEEQARP 420
EYEARCTHVV INEITQEEER IPFTAWGKCI RITLCASAVF FWILLIIASV IGIIVYRLSV 480
FIVFSAKLPK NINGTDPIQK YLTPQTATSI TASIISFIII MILNTIYEKV AIMITNFELP 540
RTQTDYENSL TMKMFLFQFV NYYSSCFYIA FFKGKFVGYP GDPVYWLGKY RNEECDPGGC 600
LLELTTQLTI IMGGKAIWNN IQEVLLPWIM NLIGRFHRVS GSEKITPRWE QDYHLQPMGK 660
LGLFYEYLEM IIQFGFVTLF VASFPLAPLL ALVNNILEIR VDAWKLTTQF RRLVPEKAQD 720
IGAWQPIMQG IAILAVVTNA MIIAFTSDMI PRLVYYWSFS VPPYGDHTSY TMEGYINNTL 780
SIFKVADFKN KSKGNPYSDL GNHTTCRYRD FRYPPGHPQE YKHNIYYWHV IAAKLAFIIV 840
MEHVIYSVKF FISYAIPDVS KRTKSKIQRE KYLTQKLLHE NHLKDMTKNM GVIAERMIEA 900
VDNNLRPKSE 910 
Gene Ontology
 GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
 GO:0005622; C:intracellular; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0005227; F:calcium activated cation channel activity; ISS:UniProtKB.
 GO:0005254; F:chloride channel activity; IEA:UniProtKB-KW.
 GO:0005244; F:voltage-gated ion channel activity; ISS:UniProtKB.
 GO:0002543; P:activation of blood coagulation via clotting cascade; IMP:UniProtKB.
 GO:0007596; P:blood coagulation; ISS:UniProtKB.
 GO:0097045; P:phosphatidylserine exposure on blood platelet; IMP:UniProtKB. 
Interpro
 IPR007632; Anoctamin. 
Pfam
 PF04547; Anoctamin 
SMART
  
PROSITE
  
PRINTS