CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021228
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ectoderm-neural cortex protein 2 
Protein Synonyms/Alias
 ENC-2; Kelch-like protein 25 
Gene Name
 KLHL25 
Gene Synonyms/Alias
 ENC2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
226KHDLEPRKVHLPELLubiquitination[1]
263LMADERTKLIMDEALubiquitination[1]
318YQVDHKAKEIIPKADubiquitination[1]
323KAKEIIPKADLPSPRubiquitination[2]
331ADLPSPRKEFSASAIubiquitination[1]
461IHRDMVSKVQCYDPSubiquitination[1]
475SENRWTIKAECPQPWubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
  
Sequence Annotation
 DOMAIN 46 114 BTB.
 REPEAT 296 340 Kelch 1.
 REPEAT 341 388 Kelch 2.
 REPEAT 389 444 Kelch 3.
 REPEAT 446 492 Kelch 4.
 REPEAT 494 538 Kelch 5.
 REPEAT 539 585 Kelch 6.  
Keyword
 Complete proteome; Kelch repeat; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 589 AA 
Protein Sequence
MSVSVHETRK SRSSTGSMNV TLFHKASHPD CVLAHLNTLR KHCMFTDVTL WAGDRAFPCH 60
RAVLAASSRY FEAMFSHGLR ESRDDTVNFQ DNLHPEVLEL LLDFAYSSRI AINEENAESL 120
LEAGDMLQFH DVRDAAAEFL EKNLFPSNCL GMMLLSDAHQ CRRLYEFSWR MCLVHFETVR 180
QSEDFNSLSK DTLLDLISSD ELETEDERVV FEAILQWVKH DLEPRKVHLP ELLRSVRLAL 240
LPSDCLQEAV SSEALLMADE RTKLIMDEAL RCKTRILQND GVVTSPCARP RKAGHTLLIL 300
GGQTFMCDKI YQVDHKAKEI IPKADLPSPR KEFSASAIGC KVYVTGGRGS ENGVSKDVWV 360
YDTVHEEWSK AAPMLIARFG HGSAELENCL YVVGGHTSLA GVFPASPSVS LKQVEKYDPG 420
ANKWMMVAPL RDGVSNAAVV SAKLKLFVFG GTSIHRDMVS KVQCYDPSEN RWTIKAECPQ 480
PWRYTAAAVL GSQIFIMGGD TEFTAASAYR FDCETNQWTR IGDMTAKRMS CHALASGNKL 540
YVVGGYFGTQ RCKTLDCYDP TSDTWNCITT VPYSLIPTAF VSTWKHLPA 589 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:LIFEdb. 
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR017096; Kelch-like_gigaxonin.
 IPR015915; Kelch-typ_b-propeller.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS