CPLM-023083

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-023083

UniProt Accession

FBXL5_HUMAN; Q9UKA1; A8MSK4; B4DIB5; Q4W5A8; Q8NHP3; Q9NXN2; Q9P0I0; Q9P0X5; Q9UJT7; Q9UKC8

Genbank Protein ID

AF176700; AF199420; AF142481; AK000153; AK295510; BX537957; AC114744; AC116651; CH471069; CH471069; BC030656; AF157323; AF174591

Genbank Nucleotide ID

AAF03700.1; AAF09249.1; AAF66616.1; BAA90978.1; BAG58427.1; CAD97924.1; AAY40929.1; EAW92737.1; EAW92739.1; AAH30656.1; AAF67489.1; AAF04512.1

Protein Name

F-box/LRR-repeat protein 5

Protein Synonyms/Alias

F-box and leucine-rich repeat protein 5; F-box protein FBL4/FBL5; p45SKP2-like protein

Gene Name

FBXL5

Gene Synonyms/Alias

FBL4; FBL5; FLR1

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
77	EMLSLFEKGLKNVKN	ubiquitination	[1]
259	EPDDEWVKNRKDESR	ubiquitination	[1]
386	ITDVALEKISRALGI	ubiquitination	[1]

Reference

[1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued.

Sequence Annotation

DOMAIN 202 248 F-box.
REPEAT 340 364 LRR 1.
REPEAT 365 392 LRR 2.
REPEAT 393 418 LRR 3.
REPEAT 479 508 LRR 4.
REPEAT 576 607 LRR 5.
REPEAT 608 635 LRR 6.
REPEAT 636 661 LRR 7.
REGION 1 159 Hemerythrin-like.
METAL 15 15 Iron 1.
METAL 57 57 Iron 1.
METAL 58 58 Iron 1.
METAL 61 61 Iron 1.
METAL 61 61 Iron 2.
METAL 80 80 Iron 2.
METAL 126 126 Iron 2.
METAL 130 130 Iron 1.
METAL 130 130 Iron 2.

Keyword

3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Iron; Leucine-rich repeat; Metal-binding; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

691 AA

Protein Sequence

MAPFPEEVDV FTAPHWRMKQ LVGLYCDKSL YATFKEFKMH EQIENEYIIG LLQQRSQTIY 60
NVHSDNKLSE MLSLFEKGLK NVKNEYEQLN YAKQLKERLE AFTRDFLPHM KEEEEVFQPM 120
LMEYFTYEEL KDIKKKVIAQ HCSQKDTAEL LRGLSLWNHA EERQKFFKYS VDEKSDKEAE 180
VSEHSTGITH LPPEVMLSIF SYLNPQELCR CSQVSMKWSQ LTKTGSLWKH LYPVHWARGD 240
WYSGPATELD TEPDDEWVKN RKDESRAFHE WDEDADIDES EESAEESIAI SIAQMEKRLL 300
HGLIHNVLPY VGTSVKTLVL AYSSAVSSKM VRQILELCPN LEHLDLTQTD ISDSAFDSWS 360
WLGCCQSLRH LDLSGCEKIT DVALEKISRA LGILTSHQSG FLKTSTSKIT STAWKNKDIT 420
MQSTKQYACL HDLTNKGIGE EIDNEHPWTK PVSSENFTSP YVWMLDAEDL ADIEDTVEWR 480
HRNVESLCVM ETASNFSCST SGCFSKDIVG LRTSVCWQQH CASPAFAYCG HSFCCTGTAL 540
RTMSSLPESS AMCRKAARTR LPRGKDLIYF GSEKSDQETG RVLLFLSLSG CYQITDHGLR 600
VLTLGGGLPY LEHLNLSGCL TITGAGLQDL VSACPSLNDE YFYYCDNING PHADTASGCQ 660
NLQCGFRACC RSGE 674

Gene Ontology

GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
GO:0019005; C:SCF ubiquitin ligase complex; IDA:UniProtKB.
GO:0005506; F:iron ion binding; IDA:UniProtKB.
GO:0004842; F:ubiquitin-protein ligase activity; NAS:UniProtKB.
GO:0055072; P:iron ion homeostasis; IMP:UniProtKB.
GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB.

Interpro

IPR001810; F-box_dom_cyclin-like.
IPR012312; Haemerythrin/HHE_cat-bd_motif.
IPR006553; Leu-rich_rpt_Cys-con_subtyp.

Pfam

PF01814; Hemerythrin

SMART

SM00256; FBOX
SM00367; LRR_CC

PROSITE

PS50181; FBOX

PRINTS