UniProt Accession

 ITA4_HUMAN; P13612; D3DPG4 

Genbank Protein ID

 X16983; L12002; AC020595; CH471058; CH471058 

Genbank Nucleotide ID

 CAA34852.1; AAB59613.1; EAX10985.1; EAX10987.1 

Protein Name

 Integrin alpha-4 

Protein Synonyms/Alias

 CD49 antigen-like family member D; Integrin alpha-IV; VLA-4 subunit alpha; CD49d 

Gene Name

 ITGA4 

Gene Synonyms/Alias

 CD49D 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
95	IYRCRIGKNPGQTCE	ubiquitination	[1, 2]
116	PNGEPCGKTCLEERD	ubiquitination	[1, 2]
149	VTCGHRWKNIFYIKN	ubiquitination	[2]
155	WKNIFYIKNENKLPT	ubiquitination	[2]
159	FYIKNENKLPTGGCY	ubiquitination	[2]
189	PCYQDYVKKFGENFA	ubiquitination	[1, 2]
190	CYQDYVKKFGENFAS	ubiquitination	[2]
236	NITTNKYKAFLDKQN	ubiquitination	[2]
241	KYKAFLDKQNQVKFG	ubiquitination	[2]
280	PQHEQIGKAYIFSID	ubiquitination	[2]
289	YIFSIDEKELNILHE	ubiquitination	[2]
298	LNILHEMKGKKLGSY	ubiquitination	[2]
300	ILHEMKGKKLGSYFG	ubiquitination	[2]
363	TNLVGSDKYAARFGE	ubiquitination	[2]
590	LGPHVISKRSTEEFP	ubiquitination	[2]
606	LQPILQQKKEKDIMK	ubiquitination	[1]
607	QPILQQKKEKDIMKK	ubiquitination	[2]
614	KEKDIMKKTINFARF	ubiquitination	[2]
641	SAKIGFLKPHENKTY	ubiquitination	[2]
646	FLKPHENKTYLAVGS	ubiquitination	[2]
760	EEEMDNLKHSRVTVA	ubiquitination	[2]
771	VTVAIPLKYEVKLTV	ubiquitination	[2]
867	VCALEQQKSAMQTLK	ubiquitination	[1, 2, 3, 4]
1010	GFFKRQYKSILQEEN	ubiquitination	[2, 4]
1028	SWSYINSKSNDD***	ubiquitination	[1, 2, 3, 4]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. 

Sequence Annotation

 REPEAT 35 100 FG-GAP 1.
 REPEAT 110 177 FG-GAP 2.
 REPEAT 185 237 FG-GAP 3.
 REPEAT 238 293 FG-GAP 4.
 REPEAT 294 351 FG-GAP 5.
 REPEAT 355 412 FG-GAP 6.
 REPEAT 416 478 FG-GAP 7.
 MOTIF 606 616 SG1.
 MOTIF 1003 1007 GFFKR motif.
 MOD_RES 1021 1021 Phosphoserine.
 CARBOHYD 79 79 N-linked (GlcNAc...) (Potential).
 CARBOHYD 138 138 N-linked (GlcNAc...) (Potential).
 CARBOHYD 229 229 N-linked (GlcNAc...) (Potential).
 CARBOHYD 480 480 N-linked (GlcNAc...).
 CARBOHYD 518 518 N-linked (GlcNAc...).
 CARBOHYD 538 538 N-linked (GlcNAc...).
 CARBOHYD 626 626 N-linked (GlcNAc...) (Potential).
 CARBOHYD 645 645 N-linked (GlcNAc...).
 CARBOHYD 660 660 N-linked (GlcNAc...) (Potential).
 CARBOHYD 806 806 N-linked (GlcNAc...) (Potential).
 CARBOHYD 821 821 N-linked (GlcNAc...) (Potential).
 DISULFID 91 101 By similarity.
 DISULFID 144 165 By similarity.
 DISULFID 183 198 By similarity.
 DISULFID 486 495 By similarity.
 DISULFID 501 557 By similarity.
 DISULFID 622 627 By similarity.
 DISULFID 698 711 By similarity.
 DISULFID 852 890 By similarity.
 DISULFID 897 902 By similarity.  

Keyword

 3D-structure; Calcium; Cell adhesion; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Integrin; Membrane; Metal-binding; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1032 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IDA:BHF-UCL.
 GO:0009897; C:external side of plasma membrane; IEA:Compara.
 GO:0008305; C:integrin complex; IEA:InterPro.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0030183; P:B cell differentiation; IC:BHF-UCL.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0001974; P:blood vessel remodeling; IEA:Compara.
 GO:0060710; P:chorio-allantoic fusion; IEA:Compara.
 GO:0060324; P:face development; IEA:Compara.
 GO:0007507; P:heart development; IEA:Compara.
 GO:0007157; P:heterophilic cell-cell adhesion; IEA:Compara.
 GO:0007229; P:integrin-mediated signaling pathway; IEA:UniProtKB-KW.
 GO:0007159; P:leukocyte cell-cell adhesion; IDA:BHF-UCL.
 GO:0050900; P:leukocyte migration; TAS:Reactome.
 GO:0050776; P:regulation of immune response; TAS:Reactome. 

Interpro

 IPR013517; FG-GAP.
 IPR013519; Int_alpha_beta-p.
 IPR000413; Integrin_alpha.
 IPR013649; Integrin_alpha-2.
 IPR018184; Integrin_alpha_C_CS. 

Pfam

 PF01839; FG-GAP
 PF08441; Integrin_alpha2 

SMART

 SM00191; Int_alpha 

PROSITE

 PS51470; FG_GAP
 PS00242; INTEGRIN_ALPHA 

PRINTS

 PR01185; INTEGRINA.