CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007377
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein GVP36 
Protein Synonyms/Alias
 36 kDa Golgi vesicle protein 
Gene Name
 GVP36 
Gene Synonyms/Alias
 YIL041W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
13FASSLSKKLQEISTSubiquitination[1, 2]
24ISTSVSEKTQELPSLubiquitination[2]
62EYTELEDKVDTIKLIubiquitination[2]
140TLNYALSKVALNSSEubiquitination[2]
173QFSDVQAKIAQARIQubiquitination[2]
188QDTLIQTKFNKNLREacetylation[3]
203RLSFEIGKADKCRKDacetylation[3]
305FAEEPEAKPEVAEEEubiquitination[1, 2, 4, 5, 6]
313PEVAEEEKPQTAISMubiquitination[1, 2, 4, 5, 6]
Reference
 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [4] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [5] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [6] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 
Functional Description
  
Sequence Annotation
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 319 319 Phosphoserine.
 CROSSLNK 305 305 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 313 313 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Golgi apparatus; Isopeptide bond; Membrane; Phosphoprotein; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 326 AA 
Protein Sequence
MSFNAFASSL SKKLQEISTS VSEKTQELPS LAQSTQRMVQ ERLGQVTDIS QLPREYTELE 60
DKVDTIKLIY NHFLGVTAIY ENGSYDYPKY INESVNEFSR SVASKLTELT HATSASEAQN 120
ILVAPGPIKE PKTLNYALSK VALNSSECLN KMFPTEEQPL ASALLQFSDV QAKIAQARIQ 180
QDTLIQTKFN KNLRERLSFE IGKADKCRKD VHSMRLRYDV ARTNLANNKK PEKEASLRVQ 240
METLEDQFAQ VTEDATVCLQ EVISHANFSE DLKELAKAQA EYFETSAGLM KEFLSNSFAE 300
EPEAKPEVAE EEKPQTAISM NDEDDA 326 
Gene Ontology
 GO:0030173; C:integral to Golgi membrane; IDA:SGD.
 GO:0006897; P:endocytosis; IMP:SGD.
 GO:0030950; P:establishment or maintenance of actin cytoskeleton polarity; IMP:SGD.
 GO:0007033; P:vacuole organization; IMP:SGD. 
Interpro
 IPR027267; AH/BAR-dom.
 IPR018859; BAR_dom-cont. 
Pfam
 PF10455; BAR_2 
SMART
  
PROSITE
  
PRINTS