UniProt Accession

 DEST_MOUSE; Q9R0P5 

Genbank Protein ID

 AB025406; AK078898 

Genbank Nucleotide ID

 BAA84691.1; BAC37447.1 

Protein Name

 Destrin 

Protein Synonyms/Alias

 Actin-depolymerizing factor; ADF; Sid 23 

Gene Name

 Dstn 

Gene Synonyms/Alias

 Dsn; Sid23 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
19	CRIFYDMKVRKCSTP	ubiquitination	[1]
30	CSTPEEIKKRKKAVI	acetylation	[2]
44	IFCLSADKKCIVVEE	acetylation	[2]
44	IFCLSADKKCIVVEE	ubiquitination	[1]
78	FVGMLPEKDCRYALY	acetylation	[3, 4]
78	FVGMLPEKDCRYALY	ubiquitination	[1]
92	YDASFETKESRKEEL	acetylation	[2, 4]
92	YDASFETKESRKEEL	ubiquitination	[1]
112	APEQAPLKSKMIYAS	ubiquitination	[1]
127	SKDAIKKKFPGIKHE	acetylation	[2, 3]
132	KKKFPGIKHEYQANG	acetylation	[2]
132	KKKFPGIKHEYQANG	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Actin-depolymerizing protein. Severs actin filaments (F- actin) and binds to actin monomers (G-actin). Acts in a pH- independent manner. 

Sequence Annotation

 DOMAIN 4 153 ADF-H.
 MOTIF 30 34 Nuclear localization signal (Potential).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 3 3 Phosphoserine.
 MOD_RES 19 19 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Actin-binding; Complete proteome; Direct protein sequencing; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 165 AA 

Protein Sequence

Gene Ontology

 GO:0030864; C:cortical actin cytoskeleton; IDA:MGI.
 GO:0030042; P:actin filament depolymerization; IEA:InterPro.
 GO:0006928; P:cellular component movement; IMP:MGI.
 GO:0000910; P:cytokinesis; IMP:MGI.
 GO:0030836; P:positive regulation of actin filament depolymerization; IMP:MGI. 

Interpro

 IPR002108; Actin-bd_cofilin/tropomyosin.
 IPR017904; ADF/Cofilin/Destrin. 

Pfam

 PF00241; Cofilin_ADF 

SMART

 SM00102; ADF 

PROSITE

 PS51263; ADF_H 

PRINTS

 PR00006; COFILIN.