CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020088
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleoside diphosphate-linked moiety X motif 22 
Protein Synonyms/Alias
 Nudix motif 22 
Gene Name
 NUDT22 
Gene Synonyms/Alias
 PP11246 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
50AIWETRLKAQPWLFDubiquitination[1, 2, 3]
60PWLFDAPKFRLHSATubiquitination[1, 2, 3, 4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
  
Sequence Annotation
 DOMAIN 118 285 Nudix hydrolase.  
Keyword
 Alternative splicing; Complete proteome; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 303 AA 
Protein Sequence
MDPEVTLLLQ CPGGGLPQEQ IQAELSPAHD RRPLPGGDEA ITAIWETRLK AQPWLFDAPK 60
FRLHSATLAP IGSRGPQLLL RLGLTSYRDF LGTNWSSSAA WLRQQGATDW GDTQAYLADP 120
LGVGAALATA DDFLVFLRRS RQVAEAPGLV DVPGGHPEPQ ALCPGGSPQH QDLAGQLVVH 180
ELFSSVLQEI CDEVNLPLLT LSQPLLLGIA RNETSAGRAS AEFYVQCSLT SEQVRKHYLS 240
GGPEAHESTG IFFVETQNVQ RLLETEMWAE LCPSAKGAII LYNRVQGSPT GAALGSPALL 300
PPL 303 
Gene Ontology
 GO:0016787; F:hydrolase activity; IEA:InterPro. 
Interpro
 IPR000086; NUDIX_hydrolase_dom. 
Pfam
  
SMART
  
PROSITE
 PS51462; NUDIX
 PS00893; NUDIX_BOX 
PRINTS