UniProt Accession

 NEK2_HUMAN; P51955; Q53FD6; Q5I1Z9; Q5VXZ1; Q6NZX8; Q7Z634; Q86XH2; Q96QN9 

Genbank Protein ID

 Z29066; AY045701; U11050; BT019729; AK223353; AL356310; AC096637; BC043502; BC052807; BC065932; Z25425; AY863109 

Genbank Nucleotide ID

 CAA82309.1; AAK92212.1; AAA19558.1; AAV38534.1; BAD97073.1; CAH72901.1; CAH72901.1; AAH43502.2; AAH52807.1; AAH65932.1; CAA80912.1; AAW56418.1 

Protein Name

 Serine/threonine-protein kinase Nek2 

Protein Synonyms/Alias

 HSPK 21; Never in mitosis A-related kinase 2; NimA-related protein kinase 2; NimA-like protein kinase 1 

Gene Name

 NEK2 

Gene Synonyms/Alias

 NEK2A; NLK1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
37	DGKILVWKELDYGSM	ubiquitination	[1]
63	VNLLRELKHPNIVRY	ubiquitination	[1]
143	TVLHRDLKPANVFLD	ubiquitination	[1, 2]
152	ANVFLDGKQNVKLGD	ubiquitination	[1, 2]
156	LDGKQNVKLGDFGLA	ubiquitination	[1, 2]
174	NHDTSFAKTFVGTPY	ubiquitination	[1]
227	SQKELAGKIREGKFR	ubiquitination	[1]
295	RQLGEPEKSQDSSPV	ubiquitination	[1]
309	VLSELKLKEIQLQER	ubiquitination	[1]
330	REERLEQKEQELCVR	ubiquitination	[1]
344	RERLAEDKLARAENL	ubiquitination	[1]
353	ARAENLLKNYSLLKE	ubiquitination	[1]
359	LKNYSLLKERKFLSL	ubiquitination	[1]
362	YSLLKERKFLSLASN	ubiquitination	[1, 3, 4]
381	NLPSSVIKKKVHFSG	ubiquitination	[5]
382	LPSSVIKKKVHFSGE	ubiquitination	[1]
391	VHFSGESKENIMRSE	ubiquitination	[1, 2, 5]
407	SESQLTSKSKCKDLK	ubiquitination	[1, 2, 5]
437	IEKNYQLKSRQILGM	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Isoform 1 phosphorylates and activates NEK11 in G1/S- arrested cells. Isoform 2, which is not present in the nucleolus, does not. 

Sequence Annotation

 DOMAIN 8 271 Protein kinase.
 NP_BIND 14 22 ATP (By similarity).
 REGION 264 445 Interaction with PCNT.
 REGION 306 334 Leucine-zipper.
 REGION 329 445 Necessary for interaction with MAD1L1.
 REGION 333 370 Required for microtubule binding and for
 REGION 403 439 Interaction with SAV1 and STK3/MST2.
 ACT_SITE 141 141 Proton acceptor (By similarity).
 BINDING 37 37 ATP.
 MOD_RES 170 170 Phosphothreonine; by autocatalysis
 MOD_RES 171 171 Phosphoserine; by autocatalysis
 MOD_RES 175 175 Phosphothreonine; by autocatalysis.
 MOD_RES 179 179 Phosphothreonine; by autocatalysis.
 MOD_RES 241 241 Phosphoserine; by autocatalysis.
 MOD_RES 296 296 Phosphoserine.
 MOD_RES 356 356 Phosphoserine; by STK3/MST2.
 MOD_RES 365 365 Phosphoserine; by STK3/MST2.
 MOD_RES 387 387 Phosphoserine.
 MOD_RES 390 390 Phosphoserine.
 MOD_RES 397 397 Phosphoserine.
 MOD_RES 402 402 Phosphoserine.
 MOD_RES 406 406 Phosphoserine; by STK3/MST2.
 MOD_RES 428 428 Phosphoserine.
 MOD_RES 438 438 Phosphoserine; by STK3/MST2.  

Keyword

 3D-structure; Alternative splicing; ATP-binding; Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Kinetochore; Magnesium; Meiosis; Metal-binding; Microtubule; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 445 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0000794; C:condensed nuclear chromosome; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000776; C:kinetochore; IDA:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0030496; C:midbody; IEA:Compara.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; TAS:UniProtKB.
 GO:0001824; P:blastocyst development; IEA:Compara.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0051299; P:centrosome separation; IDA:UniProtKB.
 GO:0007059; P:chromosome segregation; IDA:UniProtKB.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007126; P:meiosis; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; TAS:ProtInc.
 GO:0000070; P:mitotic sister chromatid segregation; IEA:Compara.
 GO:0043392; P:negative regulation of DNA binding; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB.
 GO:0051988; P:regulation of attachment of spindle microtubules to kinetochore; IMP:UniProtKB.
 GO:0046602; P:regulation of mitotic centrosome separation; TAS:UniProtKB.
 GO:0051225; P:spindle assembly; TAS:UniProtKB.
 GO:0090307; P:spindle assembly involved in mitosis; IEA:Compara. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS