UniProt Accession

 LETM1_MOUSE; Q9Z2I0; Q5PQC5; Q7TMK8; Q80ZX6; Q8CGJ3; Q8K5E5 

Genbank Protein ID

 AF061026; BC023862; BC046326; BC055865; BC061115; AB041582 

Genbank Nucleotide ID

 AAD13139.1; AAH23862.1; AAH46326.1; AAH55865.1; AAH61115.1; BAB93544.1 

Protein Name

 LETM1 and EF-hand domain-containing protein 1, mitochondrial 

Protein Synonyms/Alias

 Leucine zipper-EF-hand-containing transmembrane protein 1 

Gene Name

 Letm1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
283	AAKGNATKDFSAFFQ	acetylation	[1]
291	DFSAFFQKIRETGER	acetylation	[1]
291	DFSAFFQKIRETGER	ubiquitination	[2]
458	VAEVEGEKVDNKAKL	acetylation	[1, 3]
586	SEDLQEIKKELSKTG	acetylation	[1, 4]
596	LSKTGEEKYIEESAA	acetylation	[1, 3, 5, 6]
605	IEESAASKRLSKRVQ	acetylation	[3]
660	ISAMKQIKHIPEHKL	acetylation	[1]
722	KEEKIEEKEKAKEKA	acetylation	[1]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [6] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199] 

Functional Description

 Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization (By similarity). 

Sequence Annotation

 DOMAIN 149 417 LETM1.
 DOMAIN 662 697 EF-hand.
 MOD_RES 596 596 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Calcium; Coiled coil; Complete proteome; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Transit peptide; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 738 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0042407; P:cristae formation; IEA:Compara. 

Interpro

 IPR018247; EF_Hand_1_Ca_BS.
 IPR011685; LETM1. 

Pfam

 PF07766; LETM1 

SMART

  

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS