CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008075
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Casein kinase I isoform alpha 
Protein Synonyms/Alias
 CKI-alpha; CK1 
Gene Name
 CSNK1A1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MASSSGSKAEFIVGGacetylation[1]
8MASSSGSKAEFIVGGubiquitination[2]
51AVKLESQKARHPQLLubiquitination[3]
62PQLLYESKLYKILQGubiquitination[2, 3, 4]
65LYESKLYKILQGGVGubiquitination[2, 4]
138NFIHRDIKPDNFLMGubiquitination[2, 3]
152GIGRHCNKLFLIDFGubiquitination[2]
162LIDFGLAKKYRDNRTubiquitination[2, 3]
163IDFGLAKKYRDNRTRubiquitination[2]
179HIPYREDKNLTGTARubiquitination[2]
225SLPWQGLKAATKKQKubiquitination[2, 3, 4]
240YEKISEKKMSTPVEVubiquitination[2]
302TFDWTMLKQKAAQQAubiquitination[2, 3]
304DWTMLKQKAAQQAASubiquitination[2, 3]
325QAQTPTGKQTDKTKSubiquitination[2, 3]
329PTGKQTDKTKSNMKGubiquitination[2]
331GKQTDKTKSNMKGF*ubiquitination[2]
335DKTKSNMKGF*****ubiquitination[2]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. 
Sequence Annotation
 DOMAIN 17 285 Protein kinase.
 NP_BIND 23 31 ATP (By similarity).
 ACT_SITE 136 136 Proton acceptor (By similarity).
 BINDING 46 46 ATP (By similarity).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 8 8 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; ATP-binding; Cell cycle; Cell division; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Kinase; Kinetochore; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 337 AA 
Protein Sequence
MASSSGSKAE FIVGGKYKLV RKIGSGSFGD IYLAINITNG EEVAVKLESQ KARHPQLLYE 60
SKLYKILQGG VGIPHIRWYG QEKDYNVLVM DLLGPSLEDL FNFCSRRFTM KTVLMLADQM 120
ISRIEYVHTK NFIHRDIKPD NFLMGIGRHC NKLFLIDFGL AKKYRDNRTR QHIPYREDKN 180
LTGTARYASI NAHLGIEQSR RDDMESLGYV LMYFNRTSLP WQGLKAATKK QKYEKISEKK 240
MSTPVEVLCK GFPAEFAMYL NYCRGLRFEE APDYMYLRQL FRILFRTLNH QYDYTFDWTM 300
LKQKAAQQAA SSSGQGQQAQ TPTGKQTDKT KSNMKGF 337 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016607; C:nuclear speck; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007166; P:cell surface receptor signaling pathway; TAS:ProtInc.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS