UniProt Accession

 RUNX2_HUMAN; Q13950; O14614; O14615; O95181 

Genbank Protein ID

 AF001450; AF001443; AF001444; AF001445; AF001446; AF001447; AF001448; AF001449; AF001450; AF001444; AF001445; AF001446; AF001447; AF001448; AF001449; AL161907; AL096865; AL358135; AL161907; AL096865; AL358135; AL358135; AL096865; AL161907; AL358135; AL096865; AL161907; AL096865; AL161907; AL358135; AL096865; AL161907; AL358135; AF053952; AF053949; L40992 

Genbank Nucleotide ID

 AAB65159.2; AAB65159.2; AAB65159.2; AAB65159.2; AAB65159.2; AAB65159.2; AAB65159.2; AAB65159.2; AAB65158.1; AAB65158.1; AAB65158.1; AAB65158.1; AAB65158.1; AAB65158.1; AAB65158.1; CAI19638.1; CAI19638.1; CAI19638.1; CAI19639.1; CAI19639.1; CAI19639.1; CAI13528.1; CAI13528.1; CAI13528.1; CAI13531.1; CAI13531.1; CAI13531.1; CAI19925.1; CAI19925.1; CAI19925.1; CAI19931.1; CAI19931.1; CAI19931.1; AAC78624.1; AAC77441.1; AAA89072.1 

Protein Name

 Runt-related transcription factor 2 

Protein Synonyms/Alias

 Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit 

Gene Name

 RUNX2 

Gene Synonyms/Alias

 AML3; CBFA1; OSF2; PEBP2A 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
233	EPRRHRQKLDDSKPS	ubiquitination	[1, 2]
238	RQKLDDSKPSLFSDR	ubiquitination	[1, 2]
358	LWPSTLSKKSQAGAS	ubiquitination	[1, 2]
359	WPSTLSKKSQAGASE	ubiquitination	[1, 2]

Reference

 [1] Bone morphogenetic protein-2 stimulates Runx2 acetylation.
 Jeon EJ, Lee KY, Choi NS, Lee MH, Kim HN, Jin YH, Ryoo HM, Choi JY, Yoshida M, Nishino N, Oh BC, Lee KS, Lee YH, Bae SC.
 J Biol Chem. 2006 Jun 16;281(24):16502-11. [PMID: 16613856]
 [2] Post-translational Regulation of Runx2 in Bone and Cartilage.
 Jonason JH, Xiao G, Zhang M, Xing L, Chen D.
 J Dent Res. 2009 Aug;88(8):693-703. [PMID: 19734454] 

Functional Description

 Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation. 

Sequence Annotation

 DOMAIN 101 229 Runt.
 REGION 242 258 Required for interaction with FOXO1 (By
 REGION 336 439 Interaction with KAT6A (By similarity).
 REGION 374 468 Interaction with KAT6B.
 MOD_RES 451 451 Phosphoserine; by CDK1.  

Keyword

 Alternative splicing; Complete proteome; Disease mutation; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 521 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0000790; C:nuclear chromatin; ISS:BHF-UCL.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; NAS:ProtInc.
 GO:0044212; F:transcription regulatory region DNA binding; IEA:Compara.
 GO:0030509; P:BMP signaling pathway; ISS:BHF-UCL.
 GO:0048469; P:cell maturation; IEA:Compara.
 GO:0071773; P:cellular response to BMP stimulus; ISS:BHF-UCL.
 GO:0002063; P:chondrocyte development; IEA:Compara.
 GO:0048701; P:embryonic cranial skeleton morphogenesis; IEA:Compara.
 GO:0001958; P:endochondral ossification; IEA:Compara.
 GO:0045879; P:negative regulation of smoothened signaling pathway; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0042475; P:odontogenesis of dentin-containing tooth; IEA:Compara.
 GO:0002076; P:osteoblast development; IEA:Compara.
 GO:0001649; P:osteoblast differentiation; IEP:UniProtKB.
 GO:0002051; P:osteoblast fate commitment; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0032332; P:positive regulation of chondrocyte differentiation; IEA:Compara.
 GO:0045669; P:positive regulation of osteoblast differentiation; IEA:Compara.
 GO:1901522; P:positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus; ISS:BHF-UCL.
 GO:0040036; P:regulation of fibroblast growth factor receptor signaling pathway; IEA:Compara.
 GO:0042487; P:regulation of odontogenesis of dentin-containing tooth; IEA:Compara.
 GO:0048863; P:stem cell differentiation; IEA:Compara.
 GO:0030217; P:T cell differentiation; IEA:Compara.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome. 

Interpro

 IPR000040; AML1_Runt.
 IPR008967; p53-like_TF_DNA-bd.
 IPR012346; p53/RUNT-type_TF_DNA-bd.
 IPR013524; Runt_dom.
 IPR027384; Runx_central_dom.
 IPR013711; RunxI_C_dom.
 IPR016554; TF_Runt-rel_RUNX. 

Pfam

 PF00853; Runt
 PF08504; RunxI 

SMART

  

PROSITE

 PS51062; RUNT 

PRINTS

 PR00967; ONCOGENEAML1.