CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029930
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Microtubule-actin cross-linking factor 1 
Protein Synonyms/Alias
  
Gene Name
 Macf1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
1069ECEQRLLKQIQSPASubiquitination[1]
5814ELNPEEGKMVEEKYQubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
  
Sequence Annotation
  
Keyword
 Actin-binding; Calcium; Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 7353 AA 
Protein Sequence
MSSSDEETLS ERSCRSERSC RSERSYRSER SGSLSPCPPG DTLPWNLPLH EQKKRKSQDS 60
VLDPAERAVV RVADERDRVQ KKTFTKWVNK HLMKVRKHIN DLYEDLRDGH NLISLLEVLS 120
GIKLPREKGR MRFHRLQNVQ IALDFLKQRQ VKLVNIRNDD ITDGNPKLTL GLIWTIILHF 180
QISDIYISGE SGDMSAKEKL LLWTQKVTAG YTGVKCTNFS SCWSDGKMFN ALIHRYRPDL 240
VDMERVQVQS NRENLEQAFE VAERLGVTRL LDAEDVDVPS PDEKSVITYV SSIYDAFPKV 300
PEGGEGISAT EVDSRWQEYQ SRVDSLIPWI RQHTILMSDK SFPQNPVELK ALYNQYIHFK 360
ETEILAKERE KGRIKELYKL LEVWIEFGRI KLPQGYHPNH VEEEWGKLIV EMLEREKSLR 420
PAVERLELLL QIANKIQNGA LNCEEKLTLA KNTLQADAAH LESGQPVQCE SDVIMYIQEC 480
EGLIRQLQVD LQILRDEKYY QLEELAFRVM RLQDELVTLR LECTNLYRKG HFSSLELVPP 540
STLTTTHLKA EPLNKTTHSS STSWFRKPMT RTELVSISSS EDEGNLRFVY ELLSWVEEMQ 600
MKLERAEWGN DLPSVELQLE TQQHIHTSVE ELGSSVKEAR LYEGKMSQNF HTSYVETLGK 660
LETQYCKLKE TSSFRMRHLQ SLHKFVSRAT AELIWLNGKE EEELACDWSD SNPNISAKKT 720
YFSELTMELE GKQDVFRSLQ DTAEVLSLEN HPAKQTVEAY SAAVQSQLQW MKQLCLCVEQ 780
HVKENAAYFQ FFSDARDLES FLRNLQDSIK RKYTCDRSTS LSRLEDLLQD SMDEKEQLIQ 840
SKSSVASLVG RSKTIVQLKP RNPDHVLKST LSVKAICDYR QIEITICKND ECVLEDNSQR 900
TKWKVISPTG NEAMVPSVCF LIPPPNKEAI EMASRVEQSY QKVMALWHQL HINTKSLISW 960
NYLRKDLDTV QTWSLEKLRS LAPGECHQVM KNLQAHYEDF LQDSHDSALF SVADRLRIEE 1020
EVEACKAHFQ HLMKSLENED KEETLAKVYI SELKNIRLLL EECEQRLLKQ IQSPASSKTD 1080
RDARQDITLR IAEQEHTQED LQHLRSDLDA ISMKCNVFLQ QSPSGSSATT LRSELNLMVE 1140
KMDHVYGLST VYLNKLKTID VIVRSMQDAE LLVKGYEIKL SQEEAVPADL SALESHRTTL 1200
QHWLSDVKDK NSVFSVLDEE ITKAKKVAEQ LRHPASEPNL DLERYQEKGS QLQERWHRVI 1260
AQLETRQSEV ESIQEVLRDY RACHGTLIKW IEETTAQQEM MKPGQAEDSR VLSEQLSQQT 1320
ELFAEIERNQ TKLDQCQKFS QQYSTIVKDY ELQLMTYKAF VESQQKSPGK RRRMISSSDA 1380
ITQEFMDLRT RYTALVTLTT QHVKYISDAL RRLEEEEKVV EEEKQEHVEK VKDLLGWVST 1440
LARNTQGTTT SSHTSASADI EKAILEQQVL AEELTTKKEQ VSEAIKTSQI FLAKHGHKLS 1500
EGEKEQISEQ LRVLNKTYHD LCDGSANQLQ QLQSELAQQT EQKGCRAVAG VIDLGTVEIF 1560
PIFRAMQKGL IDQDTGLVLL ESQIIMSGLI DPENSEKLSL EEGLTRNFIN LPIYQQLLGL 1620
RDSLSLVSRL TGTLGSLSVV EAIEKKIISE RLGLKVLEVH LATGGFSLPP SENCINLEEA 1680
FHQGFIASSL HSELQSHLRS SKNLIDPNTA EKVGLLDLMQ RCIIHQESGL KLLPVKQLAG 1740
GMVSLKSGRK VSIFRAVQEG LIDRQVTVRL LEAQLFAGGI VDPRTGHRLT VEEAVRHNLI 1800
DQDMACAILI RQLQTGGIID TVTGDRMTID EAVTNNLVAA KIALVILESL WSFMGLLLPE 1860
SGEILPITDA LEQGIVSTEL AHKILHNRQQ IEALFLPTLT EIWSWEKATE SGILDKDLVN 1920
NLRSVCIPDM MPHIQLADSA EQSKVGFAAG KPPVSGPREE GSSHGEKLLF QLMTHSYIHA 1980
HTGQRLLLLD QELVEMLTSR DDCQVILPEV FEIQHQRLNT SEALQELYTG TISQISSAKH 2040
PRKPCESQFL SQNKDYPSQE NCTEAKGERS VVGIECSPAE SPERELFLKE QEAIIENVGS 2100
LKVINKVKLK LQRPLLGSRK EEQAETLREE NISGDPLLVE CPEESEGKDL STEKSKCQTP 2160
TKCSFTCHKE QVKTIKDIPS ETGTSLIKSQ NQMSQFQVDT SVGLRSEFKS EHDMNVNSLE 2220
KELKEELLVK DGHKQSQEGQ SVADGQTVAL EKTDTEDNAD EPALLHSSPF EDATLSTLSA 2280
QLQDGGIFNE ETGQKLLLNE AIAQGLVSSH TAVKLMGKLN MFRGFFDSQT CESLTTEEVI 2340
DEGLMDEKLL YNVLMSDKAI SGILDPRTHS LCSVKEAVAA GLLDKETATR ILEGQVITGG 2400
IVDLKRGKKL SVTLASNLGL VDTADQTELI NLEKATKGRG AEKAVKERLI ELQMETAGLM 2460
DPESKAPLTV LQSIDRGILE REAAVYLLTK QVLDGGIIHH ISGLRLSVDN AFKHGLIGED 2520
MARQLRKVEN FIHYQFFHPQ TKEALSFSEA IKLDLVSPDL KREIQEIQDF SGNLGDFIYG 2580
QKLTLAKTNK EESLANKTEL PSGVMHGVID PENCTIIPYS ELVKKCRIDT ESGWRYLEVI 2640
PFSDIKDEAG NNVLTPPEAI QLGKVDFASA LKVLEAQANT GGIIDMATGK RVTLASALEK 2700
KLLDENMARI IASHQMLSGG IIDIYSDQRV TLNDAVEKRL ISPELAAMIQ VDPLAEQGGT 2760
GVCELKGDFL RKELLSESSK TPRESYSKEK HEAVLQAGSL CAPEKAGIRG SNGEKAEKGR 2820
KISVEMEGQR QDEKASSDNK VSASILSPFG FEGESSYQVS VTHPCSESCD LKPREETRSC 2880
MKKCAVVERD KVVTQIKMVS HVKQSTSGLD AEEARERQGR MVSKEQGSHY ETAGNLLSER 2940
SVRVDRRVRR EMGGEQSVQM SREAAVLSEE ELDQEVTIGD EPDSFVKSQS MKMIGNDKGK 3000
EAGIEKDISV VCKIEGFPSQ MTSKDASLTN QDALPFYTEG ETKTVNLCSI LKPGEKLSQE 3060
TASTVQKEPL SSEIPRPERL NSQESDEEPQ ISDVPHISKG DMAAQITTRQ ETTDVQDLYI 3120
TSKSSETKDK IFPSKNYIEK LHQEIPMDPT RSHKLKEATI STLETEGISY LDSSDIKSLC 3180
EDSKADHKSC GHQKSKVTTT QAKKSLEVVD LLVRDTEEGS SEDRVGQRGP RVLASLLPEK 3240
LPTRTVQSEN IRQHDAVIPA ISEIREEMAL SLPCSVVKVD GKIPKEKHKE ILGDEQGPFM 3300
AIPSGKGIEG VNPEPCRATQ NVFTRRLCLE HDEKLVSYLS LLRDIEMRTK QIQPLELNVA 3360
ELQDLLGQAK ELDRELKDLS TVVSQELECV DRIVISQPQE VPAQLLKALE KDAKNLQKSL 3420
DSVSDSWSSR FLHLQSAVEV KKATVLNRHK ELQGKLQDLR AWVGRASLTL NSKGCDTETD 3480
ADSLSHTLQP YKDMKQSMAE RKSQLDALAL DIQLFISEHP QDLSLQQNQE MLQFLSELQR 3540
SFQGLVEHTA AQKDVVQGHL QQVQQEVQVK TLQKQQDTCH KKLEDLCNWV GQAERALERH 3600
QGGASRQELP ALQQNQSDLK DLQGDIQSHS TSFATAVKDI EGFLEENQTK LSPQELTALR 3660
EKLHQAKEQY EVLQERTRVA QKELEEAVTS ALQQETEKSK AATELAENKR KIDALLDWVA 3720
SVGSSERKPQ ASLPGMEQFS GACLEKQTLA ATDGHVDVNQ VPETLDRQYE LMKARHQELL 3780
SQQQNFIVAT QSVQSFLDQH SHNLTPEERQ KLQEKLGELK EQYAASLARS EAELKQTQAL 3840
RDELQKFLQD HKEFENWLQQ SENELDSMHK GGSSPEALNS LLKRQGSFSE DVISHKGDLR 3900
FVTISGQKVL ETENNFEEGQ EPSATRNLVN EKLKDATERY TTLHSKCIRL GSHLSMLLGQ 3960
YQQFQSSADS LQAWVLTCEA SVGKLLSDTV ASDPGVLQQQ LATTKQLQEE LAEHQVPVEK 4020
LQKAAHDLLD IEGEPALDCR PIQETTDSIS SRFQNLSCSL DERSALLQKA IAQSQSVQES 4080
MESLLQSIRE VEQNLERDQV ASLSSGVIQE ALANNMKLKQ DIARQKSSLE ATHDMVTRFM 4140
ETADSNSASV LQGKLAELSQ RFQQLQLQQQ EKESNLKKLL PQAEMFEQLS NKLQQFMENK 4200
SRLLASGNQP DQDIAHFSQQ IQELTLAMED QKENLDTLEH LVTTLGSCGF ALDLSQHQDK 4260
IQNLKKDFTE LQKTVQEREK DASTCQEQLD EFRKLIRTFQ KWLKETEGNV PPAKTFVSAK 4320
ELEKQIEHLK DLISDWESKG ALLGEINAKG TALESLIMDI TAPDSQAKTG SILPPVGSSV 4380
GSVNGYHTCK DLTEIQCDMF DVNSKYEKLW EVLRERQESL QTVFSRMEEV QKEASSVLQW 4440
LESKEEVLKA MDATLSPTKT ETVKAQAESN KAFLAELEQN SPKIQKVKEA LAGLLKTYPN 4500
SQEAENWKKM QEDLNSRWEK ATEVTVARQK QLEESASHLA CFQAAESQLR PWLMEKELMM 4560
GVLGPLSIDP NMLNAQKQQF MLKEFEARRQ QHEQLNEAAQ GILTGPGDMS PSASQVHKDL 4620
QSISQKWVEL TDKLNSRSSQ IDQAIVKSTQ YQDLLQDLSE KVKAIGQRLS GQSAISTQPE 4680
AVKQQLEETS EIRSDLGQLD NEIKEAQTLC QELSLLIGEQ YLKDELKKRL ETVALPLQGL 4740
EDLAADRMNR LQAALASTQQ FQQMFDELRT WLDEKQSQQA KNCPISAKLE RLQCQLQENE 4800
EFQKNLNQHS GSYEVIVAEG EALLLSVPPG EEKKTLQNQL VELRSHWEDL SKKTANRQSR 4860
LKDCMQKAQK YQGHVEDLVP WIDECKSKMS ELQVTLDPVQ LESSLLRSKA MLNEAEKRRS 4920
LLEILNSAAD ILINSSEIDE DEIRDEKAGL NQNMDAITEE LQAKTSSLEE MTQRLKEFQE 4980
SFKNIEKKVE GAKHQLEIFD ALGSQACSNK NLEKLKAQQE VLQALEPQVD YLRNFTQGLV 5040
EDAPDGSDAS PLVHQAEVAQ QEFLEVKQRV SSSCLTMENK LEGIGQFHCR VREMFSQLAD 5100
LDDELDGMGA IGRDTDSLQS QIEDVRLFLN KIQALRFDIE DSEAECRKML EEEGTLDLLG 5160
LKRELEALNK QCGKLTERGK VRQEQLELTL GRVEDFYRKL KALNDAATAA EEGEALQWIV 5220
GTEVDVINQQ LADFKLFQKD QVDPLQVKLQ QVNGLGQGLI QSAGKNCDVQ GLEHDMDEIN 5280
TRWNTLNKKV AQRIAQLQEA LLHCGKFQDA LEPLLSWLTD TEELIANQKP PSAEYKVVKA 5340
QIQEQKLLQR LLDDRKATVD MLQAEGGRIA QSAELADREK ITGQLESLER RWTDLLSKAA 5400
ARQKQLEDIL VLAKQFHETA EPISDFLSVT EKKLANSEPV GTQTAKIHQQ IIRHKALNEE 5460
IINRKKNVDQ AIKNGQALLK QTTGEEVLLI QEKLDGIKTR YADITLTSSK ALRTLEQARQ 5520
LATKFHSTYE ELTGWLREAE EELAASGGQS PTGEQIPQFQ QRQKELKKEV MEHRLVLDTV 5580
NEVSHALLEL VPWRAREGLD KLVSDANEQY KLISDTVGQR VDEIDAAIQR SQQYEQAADA 5640
ELAWVAETKR KLMALGPIRL EQDQTTAQLQ VQKAFSIDII RHKDSMDELF SHRGEIFSTC 5700
GEEQKAVLQE KTECLIQQYE AVSLLNSERY ARLERAQVLV NQFWETYEEL SPWAEETLAL 5760
IAQLPPPAVD HEQLRQQQEE MRQLRESIAE HKPHIDKILK IGPQLKELNP EEGKMVEEKY 5820
QKAENMYAQI KDEVRQRALA LDEAVSQSAQ FHDKIEPMLE TLENLSSRLR MPPLIPAEVD 5880
KIRECISDNK SATVELEKLQ PSFEALKRRG EELIGRSQGA DKDLAAKEIQ DKLDQMVFFW 5940
EDIKARSEER EIKFLDVLEL AEKFWYDMAA LLTTIKDTQD IVHDLESPGI DPSIIKQQVE 6000
AAETIKEETD GLHEELEFIR ILGADLIFAC GETEKPEVKK SIDEMNNAWE NLNKTWKERL 6060
EKLEDAMQAA VQYQDTLQAM FDWLDNTVIK LCTMPPVGTD LNTVKDQLNE MKEFKVEVYQ 6120
QQIEMEKLNH QGELMLKKAT DETDRDIIRE PLTELKHLWE NLGEKIAHRQ HKLEGALLAL 6180
GQFQHALEEL MSWLTHTEEL LDAQRPISGD PKVIEVELAK HHVLKNDVLA HQATVATVNK 6240
AGSELLESSA GDDASSLRSR LETMNQCWES VLQKTEEREQ QLQSTLQQAQ GFHSEIEDFL 6300
LELNRMENQL SASKPTGGLP ETAREQLDTH MELHSQLRAK EEIYNQLLDK GRLMLLSRGD 6360
SGSGSKTEQS VALLEQKWHA VSSKVEERKS KLEEALSLAT EFQNSLQEFI NWLTLAEQSL 6420
NIASPPSLIL NTVLSQIEEH KVFANEVNDH RDQIIELDQT GNQLKFLSQK QDVVLIKNLL 6480
VSVQSRWEKV VQRSIERGRS LDDARKRAKQ FHEAWKKLID WLEDAESHLD SELEISNDPD 6540
KIKLQLSKHK EFQKTLGGKQ PVYDTTIRTG RALKEKTLLA GDTQKLDNLL GEVRDKWDTV 6600
CGKSVERQHK LEEALLFSGQ FMDALQALVD WLYKVEPQLA EDQPVHGDLD LVMNLMDAHK 6660
VFQKELGKRT GTVQVLKRSG RELIEGSRDD TTWVKGQLQE LSTRWDTVCK LSVSKQSRLE 6720
QALKQAEEFR DTVHMLLEWL SEAEQTLRFR GALPDDTEAL QSLIDTHKEF MKKVEEKRVD 6780
VNTAVAMGEA ILAVCHPDCI TTIKHWITII RARFEEVLTW AKQHQQRLET ALSELVANAE 6840
LLEELLAWIQ WAETTLIQRD QEPIPQNIDR VKALITEHQS FMEEMTRKQP DVDRVTKTYK 6900
RKSVEPTHAP FMEKSRSGSR KSLNQPTPPP MPILSQSEAK NPRINQLSAR WQQVWLLALE 6960
RQRKLNDALD RLEELCPELK EFANFDFDVW RKKYMRWMNH KKSRVMDFFR RIDKDQDGKI 7020
TRQEFIDGIL ASKFPTTKLE MTAVADIFDR DGDGYIDYYE FVAALHPNKD AYRPTTDADK 7080
IEDEVTRQVA QCKCAKRFQV EQIGENKYRF FLGNQFGDSQ QLRLVRILRS TVMVRVGGGW 7140
MALDEFLVKN DPCRARGRTN IELREKFILP EGASQGMTPF RSRGRRSKPS SRAASPTRSS 7200
SSASQSNHSC TSMPSSPATP ASGTKVISSS GSKLKRPTPA FHSSRTSLAG DTSNSSSPAS 7260
TGAKANRADP KKSASRPGSR AGSRAGSRAS SRRGSDASDF DLLETQSACS DTSESSAAGG 7320
QGSSRRGLTK PSKIPTMSKK TTTASPRTPG PKR 7353 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IDA:MGI.
 GO:0015630; C:microtubule cytoskeleton; IDA:MGI.
 GO:0003779; F:actin binding; IDA:MGI.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0008017; F:microtubule binding; IDA:MGI.
 GO:0007050; P:cell cycle arrest; IEA:InterPro.
 GO:0006928; P:cellular component movement; IMP:MGI.
 GO:0007163; P:establishment or maintenance of cell polarity; IMP:MGI.
 GO:0001707; P:mesoderm formation; IMP:MGI.
 GO:0006620; P:posttranslational protein targeting to membrane; IMP:MGI.
 GO:0016055; P:Wnt receptor signaling pathway; IMP:MGI. 
Interpro
 IPR001589; Actinin_actin-bd_CS.
 IPR001715; CH-domain.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR003108; GAS2_dom.
 IPR001101; Plectin_repeat.
 IPR018159; Spectrin/alpha-actinin.
 IPR002017; Spectrin_repeat. 
Pfam
 PF00307; CH
 PF13499; EF_hand_5
 PF02187; GAS2
 PF00681; Plectin
 PF00435; Spectrin 
SMART
 SM00033; CH
 SM00054; EFh
 SM00243; GAS2
 SM00250; PLEC
 SM00150; SPEC 
PROSITE
 PS00019; ACTININ_1
 PS00020; ACTININ_2
 PS50021; CH
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS51460; GAR 
PRINTS