CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017059
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial Rho GTPase 1 
Protein Synonyms/Alias
 MIRO-1; hMiro-1; Rac-GTP-binding protein-like protein; Ras homolog gene family member T1 
Gene Name
 RHOT1 
Gene Synonyms/Alias
 ARHT1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
153ECSAKNLKNISELFYubiquitination[1]
187EMKPACIKALTRIFKubiquitination[1, 2, 3, 4]
512EYCARIFKQHFMDSRubiquitination[4]
645LLQSVKNKIFTAVLNubiquitination[1, 3]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution. 
Sequence Annotation
 DOMAIN 1 177 Miro 1.
 DOMAIN 184 219 EF-hand 1.
 DOMAIN 304 339 EF-hand 2.
 DOMAIN 412 561 Miro 2.
 NP_BIND 11 18 GTP 1.
 NP_BIND 57 61 GTP 1 (Potential).
 NP_BIND 118 121 GTP 1 (Potential).
 NP_BIND 425 432 GTP 2.
 NP_BIND 463 467 GTP 2 (Potential).
 NP_BIND 527 530 GTP 2 (Potential).  
Keyword
 Alternative splicing; Calcium; Complete proteome; GTP-binding; Hydrolase; Membrane; Metal-binding; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Reference proteome; Repeat; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 618 AA 
Protein Sequence
MKKDVRILLV GEPRVGKTSL IMSLVSEEFP EEVPPRAEEI TIPADVTPER VPTHIVDYSE 60
AEQSDEQLHQ EISQANVICI VYAVNNKHSI DKVTSRWIPL INERTDKDSR LPLILVGNKS 120
DLVEYSSMET ILPIMNQYTE IETCVECSAK NLKNISELFY YAQKAVLHPT GPLYCPEEKE 180
MKPACIKALT RIFKISDQDN DGTLNDAELN FFQRICFNTP LAPQALEDVK NVVRKHISDG 240
VADSGLTLKG FLFLHTLFIQ RGRHETTWTV LRRFGYDDDL DLTPEYLFPL LKIPPDCTTE 300
LNHHAYLFLQ STFDKHDLDR DCALSPDELK DLFKVFPYIP WGPDVNNTVC TNERGWITYQ 360
GFLSQWTLTT YLDVQRCLEY LGYLGYSILT EQESQASAVT VTRDKKIDLQ KKQTQRNVFR 420
CNVIGVKNCG KSGVLQALLG RNLMRQKKIR EDHKSYYAIN TVYVYGQEKY LLLHDISESE 480
FLTEAEIICD VVCLVYDVSN PKSFEYCARI FKQHFMDSRI PCLIVAAKSD LHEVKQEYSI 540
SPTDFCRKHK MPPPQAFTCN TADAPSKDIF VKLTTMAMYP EDHYRDRLSR DMGHTDRIEN 600
LRKIWVFLKT AFHARLRCMC TCNRCTFCIC QNFLNSDLLQ SVKNKIFTAV LNRHVTQADL 660
KSSTFWLRAS FGATVFAVLG FAMYKALLKQ R 691 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031307; C:integral to mitochondrial outer membrane; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0019725; P:cellular homeostasis; IMP:UniProtKB.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0097345; P:mitochondrial outer membrane permeabilization; IMP:UniProtKB.
 GO:0047497; P:mitochondrion transport along microtubule; IMP:UniProtKB.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR013566; EF_hand_assoc_1.
 IPR013567; EF_hand_assoc_2.
 IPR002048; EF_hand_dom.
 IPR020860; MIRO.
 IPR013684; MIRO-like.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR021181; Small_GTPase_Miro. 
Pfam
 PF08355; EF_assoc_1
 PF08356; EF_assoc_2
 PF13405; EF_hand_4
 PF08477; Miro
 PF00071; Ras 
SMART
 SM00054; EFh 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS51423; MIRO 
PRINTS
 PR00449; RASTRNSFRMNG.