UniProt Accession

 SLD5_HUMAN; Q9BRT9; B2R8H5; D3DSY0; Q8N648 

Genbank Protein ID

 AK095334; AK313373; CH471080; CH471080; BC005995; BC027454 

Genbank Nucleotide ID

 BAG53029.1; BAG36172.1; EAW63256.1; EAW63257.1; AAH05995.1; AAH27454.1 

Protein Name

 DNA replication complex GINS protein SLD5 

Protein Synonyms/Alias

 GINS complex subunit 4; DNA replication complex GINS protein SLD5, N-terminally processed 

Gene Name

 GINS4 

Gene Synonyms/Alias

 SLD5 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
102	CRLMKIEKFFPHVLE	ubiquitination	[1]
110	FFPHVLEKEKTRPEG	ubiquitination	[1]
112	PHVLEKEKTRPEGEP	ubiquitination	[1]
142	ANTESYLKNVALKHM	ubiquitination	[1]
164	DLFRAVPKPDLDSYV	ubiquitination	[1, 2, 3]
200	DYVIDLEKGSQHLIR	ubiquitination	[1]
209	SQHLIRYKTIAPLVA	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA. 

Sequence Annotation

 REGION 166 223 Important for GINS complex assembly.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 2 2 N-acetylthreonine; in DNA replication
 MOD_RES 12 12 Phosphoserine.
 MOD_RES 16 16 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; DNA replication; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 223 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0006271; P:DNA strand elongation involved in DNA replication; TAS:Reactome.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome. 

Interpro

 IPR021151; GINS_complex.
 IPR008591; GINS_Sld5. 

Pfam

 PF05916; Sld5 

SMART

  

PROSITE

  

PRINTS