CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011692
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 FCH domain only protein 2 
Protein Synonyms/Alias
  
Gene Name
 FCHO2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
58RSMTKLAKSASNYSQubiquitination[1, 2]
288GIKPRKRKTFALPGIubiquitination[3]
297FALPGIIKKEKDAESubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides (Pi(4,5) biphosphate) like the plasma membrane. Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP- 2-dependent endocytosis of the transferin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor. 
Sequence Annotation
 DOMAIN 4 85 FCH.
 DOMAIN 542 809 MHD.
 REGION 3 274 Mediates dimerization and binding to
 REGION 521 810 Mediates interaction with DAB2, EPS15,
 MOD_RES 385 385 Phosphothreonine.
 MOD_RES 387 387 Phosphoserine.
 MOD_RES 394 394 Phosphoserine.
 MOD_RES 403 403 Phosphoserine.
 MOD_RES 488 488 Phosphoserine.
 MOD_RES 509 509 Phosphoserine (By similarity).
 MOD_RES 511 511 Phosphoserine.
 MOD_RES 533 533 Phosphoserine.
 DISULFID 147 147 Interchain (with C-273).
 DISULFID 273 273 Interchain (with C-147).  
Keyword
 3D-structure; Alternative splicing; Coated pit; Coiled coil; Complete proteome; Disulfide bond; Endocytosis; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 810 AA 
Protein Sequence
MVMAYFVENF WGEKNSGFDV LYHNMKHGQI STKELADFVR ERATIEEAYS RSMTKLAKSA 60
SNYSQLGTFA PVWDVFKTST EKLANCHLDL VRKLQELIKE VQKYGEEQVK SHKKTKEEVA 120
GTLEAVQTIQ SITQALQKSK ENYNAKCVEQ ERLKKEGATQ REIEKAAVKS KKATDTYKLY 180
VEKYALAKAD FEQKMTETAQ KFQDIEETHL IHIKEIIGSL SNAIKEIHLQ IGQVHEEFIN 240
NMANTTVESL IQKFAESKGT GKERPGLIEF EECDTASAVE GIKPRKRKTF ALPGIIKKEK 300
DAESVECPDA DSLNIPDVDE EGYSIKPETN QNDTKENHFY SSSDSDSEDE EPKKYRIEIK 360
PMHPNNSHHT MASLDELKVS IGNITLSPAI SRHSPVQMNR NLSNEELTKS KPSAPPNEKG 420
TSDLLAWDPL FGPSLDSSSS SSLTSSSSAR PTTPLSVGTI VPPPRPASRP KLTSGKLSGI 480
NEIPRPFSPP VTSNTSPPPA APLARAESSS SISSSASLSA ANTPTVGVSR GPSPVSLGNQ 540
DTLPVAVALT ESVNAYFKGA DPTKCIVKIT GDMTMSFPSG IIKVFTSNPT PAVLCFRVKN 600
ISRLEQILPN AQLVFSDPSQ CDSNTKDFWM NMQAVTVYLK KLSEQNPAAS YYNVDVLKYQ 660
VSSNGIQSTP LNLATYWKCS ASTTDLRVDY KYNPEAMVAP SVLSNIQVVV PVDGGVTNMQ 720
SLPPAIWNAE QMKAFWKLSS ISEKSENGGS GSLRAKFDLS EGPSKPTTLA VQFLSEGSTL 780
SGVDFELVGT GYRLSLIKKR FATGRYLADC 810 
Gene Ontology
 GO:0030136; C:clathrin-coated vesicle; IDA:UniProtKB.
 GO:0005905; C:coated pit; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; ISS:UniProtKB.
 GO:0001786; F:phosphatidylserine binding; ISS:UniProtKB.
 GO:0048268; P:clathrin coat assembly; IMP:UniProtKB.
 GO:0072583; P:clathrin-mediated endocytosis; IMP:UniProtKB.
 GO:0010324; P:membrane invagination; ISS:UniProtKB.
 GO:0072659; P:protein localization to plasma membrane; ISS:UniProtKB. 
Interpro
 IPR001060; FCH_dom.
 IPR018808; Muniscin_C-term_mu_dom. 
Pfam
 PF00611; FCH
 PF10291; muHD 
SMART
 SM00055; FCH 
PROSITE
 PS50133; FCH
 PS51072; MHD 
PRINTS