CPLM-000327

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-000327

UniProt Accession

CSKP_HUMAN; O14936; A6NES1; B7ZKY0; O43215; Q17RI4; Q59HA0; Q5VT16; Q5VT17; Q5VT18; Q5VT19; Q66T42; Q9BYH6; Q9NYB2; Q9NYB3

Genbank Protein ID

Genbank Nucleotide ID

AAB88125.1; AAB88198.1; BAB12252.2; AAU10527.1; CAH71237.1; CAH71237.1; CAH71237.1; CAH71237.1; CAH71237.1; CAH71238.1; CAH71238.1; CAH71238.1; CAH71238.1; CAH71238.1; CAH71239.1; CAH71239.1; CAH71239.1; CAH71239.1; CAH71239.1; CAH71240.1; CAH71240.1; CAH71240.1; CAH71240.1; CAH71240.1; CAI41092.1; CAI41092.1; CAI41092.1; CAI41092.1; CAI41092.1; CAI41093.1; CAI41093.1; CAI41093.1; CAI41093.1; CAI41093.1; CAI41094.1; CAI41094.1; CAI41094.1; CAI41094.1; CAI41094.1; CAI41095.1; CAI41095.1; CAI41095.1; CAI41095.1; CAI41095.1; CAI41634.1; CAI41634.1; CAI41634.1; CAI41634.1; CAI41634.1; CAI41635.1; CAI41635.1; CAI41635.1; CAI41635.1; CAI41635.1; CAI41636.1; CAI41636.1; CAI41636.1; CAI41636.1; CAI41636.1; CAI41637.1; CAI41637.1; CAI41637.1; CAI41637.1; CAI41637.1; CAI42244.1; CAI42244.1; CAI42244.1; CAI42244.1; CAI42244.1; CAI42245.1; CAI42245.1; CAI42245.1; CAI42245.1; CAI42245.1; CAI42246.1; CAI42246.1; CAI42246.1; CAI42246.1; CAI42246.1; CAI42247.1; CAI42247.1; CAI42247.1; CAI42247.1; CAI42247.1; CAI42762.1; CAI42762.1; CAI42762.1; CAI42762.1; CAI42762.1; CAI42763.1; CAI42763.1; CAI42763.1; CAI42763.1; CAI42763.1; CAI42764.1; CAI42764.1; CAI42764.1; CAI42764.1; CAI42764.1; CAI42765.1; CAI42765.1; CAI42765.1; CAI42765.1; CAI42765.1; AAI17312.1; AAI43455.1; BAD92096.1; AAF72666.1; AAF72667.1

Protein Name

Peripheral plasma membrane protein CASK

Protein Synonyms/Alias

hCASK; Calcium/calmodulin-dependent serine protein kinase; Protein lin-2 homolog

Gene Name

CASK

Gene Synonyms/Alias

LIN2

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
41	TGQQFAVKIVDVAKF	ubiquitination	[1]
825	TKLETIRKIHEQGLI	ubiquitination	[2, 3]

Reference

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.

Sequence Annotation

DOMAIN 12 276 Protein kinase.
DOMAIN 343 398 L27 1.
DOMAIN 402 455 L27 2.
DOMAIN 489 564 PDZ.
DOMAIN 615 682 SH3.
DOMAIN 739 911 Guanylate kinase-like.
NP_BIND 18 26 ATP (By similarity).
REGION 305 315 Calmodulin-binding.
ACT_SITE 141 141 By similarity.
BINDING 41 41 ATP (By similarity).
MOD_RES 151 151 Phosphoserine; by autocatalysis.
MOD_RES 155 155 Phosphoserine; by autocatalysis.
MOD_RES 571 571 Phosphotyrosine (By similarity).

Keyword

3D-structure; Alternative splicing; ATP-binding; Calmodulin-binding; Cell membrane; Complete proteome; Cytoplasm; Disease mutation; Kinase; Membrane; Mental retardation; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Serine/threonine-protein kinase; SH3 domain; Transferase.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

926 AA

Protein Sequence

MADDDVLFED VYELCEVIGK GPFSVVRRCI NRETGQQFAV KIVDVAKFTS SPGLSTEDLK 60
REASICHMLK HPHIVELLET YSSDGMLYMV FEFMDGADLC FEIVKRADAG FVYSEAVASH 120
YMRQILEALR YCHDNNIIHR DVKPHCVLLA SKENSAPVKL GGFGVAIQLG ESGLVAGGRV 180
GTPHFMAPEV VKREPYGKPV DVWGCGVILF ILLSGCLPFY GTKERLFEGI IKGKYKMNPR 240
QWSHISESAK DLVRRMLMLD PAERITVYEA LNHPWLKERD RYAYKIHLPE TVEQLRKFNA 300
RRKLKGAVLA AVSSHKFNSF YGDPPEELPD FSEDPTSSGL LAAERAVSQV LDSLEEIHAL 360
TDCSEKDLDF LHSVFQDQHL HTLLDLYDKI NTKSSPQIRN PPSDAVQRAK EVLEEISCYP 420
ENNDAKELKR ILTQPHFMAL LQTHDVVAHE VYSDEALRVT PPPTSPYLNG DSPESANGDM 480
DMENVTRVRL VQFQKNTDEP MGITLKMNEL NHCIVARIMH GGMIHRQGTL HVGDEIREIN 540
GISVANQTVE QLQKMLREMR GSITFKIVPS YRTQSSSCER DSPSTSRQSP ANGHSSTNNS 600
VSDLPSTTQP KGRQIYVRAQ FEYDPAKDDL IPCKEAGIRF RVGDIIQIIS KDDHNWWQGK 660
LENSKNGTAG LIPSPELQEW RVACIAMEKT KQEQQASCTW FGKKKKQYKD KYLAKHNAVF 720
DQLDLVTYEE VVKLPAFKRK TLVLLGAHGV GRRHIKNTLI TKHPDRFAYP IPHTTRPPKK 780
DEENGKNYYF VSHDQMMQDI SNNEYLEYGS HEDAMYGTKL ETIRKIHEQG LIAILDVEPQ 840
ALKVLRTAEF APFVVFIAAP TITPGLNEDE SLQRLQKESD ILQRTYAHYF DLTIINNEID 900
ETIRHLEEAV ELVCTAPQWV PVSWVY 926

Gene Ontology

GO:0015629; C:actin cytoskeleton; TAS:ProtInc.
GO:0005604; C:basement membrane; IEA:Compara.
GO:0016323; C:basolateral plasma membrane; IEA:Compara.
GO:0005911; C:cell-cell junction; IDA:BHF-UCL.
GO:0060170; C:cilium membrane; ISS:BHF-UCL.
GO:0005737; C:cytoplasm; IDA:BHF-UCL.
GO:0005829; C:cytosol; IEA:Compara.
GO:0005652; C:nuclear lamina; IDA:BHF-UCL.
GO:0016363; C:nuclear matrix; IDA:BHF-UCL.
GO:0005730; C:nucleolus; IDA:BHF-UCL.
GO:0042734; C:presynaptic membrane; ISS:BHF-UCL.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0004385; F:guanylate kinase activity; TAS:ProtInc.
GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
GO:0070509; P:calcium ion import; IEA:Compara.
GO:0007155; P:cell adhesion; TAS:ProtInc.
GO:0001953; P:negative regulation of cell-matrix adhesion; IMP:BHF-UCL.
GO:0090288; P:negative regulation of cellular response to growth factor stimulus; IMP:BHF-UCL.
GO:0010839; P:negative regulation of keratinocyte proliferation; IMP:BHF-UCL.
GO:0061045; P:negative regulation of wound healing; IMP:BHF-UCL.
GO:0090280; P:positive regulation of calcium ion import; IEA:Compara.
GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.

Interpro

IPR008144; Guanylate_kin.
IPR008145; Guanylate_kin/L-typ_Ca_channel.
IPR020590; Guanylate_kinase_CS.
IPR011009; Kinase-like_dom.
IPR004172; L27.
IPR014775; L27_C.
IPR027417; P-loop_NTPase.
IPR001478; PDZ.
IPR000719; Prot_kinase_cat_dom.
IPR002290; Ser/Thr_dual-sp_kinase_dom.
IPR011511; SH3_2.
IPR001452; SH3_domain.

Pfam

PF00625; Guanylate_kin
PF02828; L27
PF00595; PDZ
PF00069; Pkinase
PF07653; SH3_2

SMART

SM00072; GuKc
SM00569; L27
SM00228; PDZ
SM00220; S_TKc
SM00326; SH3

PROSITE

PS00856; GUANYLATE_KINASE_1
PS50052; GUANYLATE_KINASE_2
PS51022; L27
PS50106; PDZ
PS00107; PROTEIN_KINASE_ATP
PS50011; PROTEIN_KINASE_DOM
PS00108; PROTEIN_KINASE_ST
PS50002; SH3

PRINTS