UniProt Accession

 IPYR_YEAST; P00817; D6VQ12 

Genbank Protein ID

 X13253; Z35880; AY692953; BK006936 

Genbank Nucleotide ID

 CAA31629.1; CAA84949.1; AAT92972.1; DAA07132.1 

Protein Name

 Inorganic pyrophosphatase 

Protein Synonyms/Alias

 Pyrophosphate phospho-hydrolase; PPase 

Gene Name

 IPP1 

Gene Synonyms/Alias

 PPA; PPA1; YBR011C; YBR0202 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
11	TTRQIGAKNTLEYKV	ubiquitination	[1]
22	EYKVYIEKDGKPVSA	acetylation	[2]
57	IPRWTNAKLEITKEE	acetylation	[2]
62	NAKLEITKEETLNPI	acetylation	[2]
62	NAKLEITKEETLNPI	ubiquitination	[1]
74	NPIIQDTKKGKLRFV	acetylation	[2]
194	NEWFRIYKIPDGKPE	acetylation	[2]
199	IYKIPDGKPENQFAF	ubiquitination	[1]
214	SGEAKNKKYALDIIK	acetylation	[2]
221	KYALDIIKETHDSWK	acetylation	[2]
228	KETHDSWKQLIAGKS	acetylation	[2]
234	WKQLIAGKSSDSKGI	ubiquitination	[1]
239	AGKSSDSKGIDLTNV	acetylation	[2]
239	AGKSSDSKGIDLTNV	ubiquitination	[1, 3]
256	PDTPTYSKAASDAIP	acetylation	[2]
256	PDTPTYSKAASDAIP	ubiquitination	[1]
275	KADAPIDKSIDKWFF	acetylation	[2]
279	PIDKSIDKWFFISGS	ubiquitination	[1, 3]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

  

Sequence Annotation

 ACT_SITE 90 90 Proton donor.
 METAL 116 116 Magnesium 1.
 METAL 121 121 Magnesium 1.
 METAL 121 121 Magnesium 2.
 METAL 153 153 Magnesium 1.
 BINDING 79 79 Diphosphate.
 MOD_RES 65 65 Phosphothreonine.
 MOD_RES 251 251 Phosphothreonine.
 MOD_RES 266 266 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Magnesium; Metal-binding; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 287 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:SGD.
 GO:0004427; F:inorganic diphosphatase activity; IDA:SGD.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0042803; F:protein homodimerization activity; IMP:SGD.
 GO:0006796; P:phosphate-containing compound metabolic process; IC:SGD. 

Interpro

 IPR008162; Pyrophosphatase. 

Pfam

 PF00719; Pyrophosphatase 

SMART

  

PROSITE

 PS00387; PPASE 

PRINTS