UniProt Accession

 UME6_YEAST; P39001; D6VSI8 

Genbank Protein ID

 L32186; L24539; D23663; Z68194; BK006938 

Genbank Nucleotide ID

 AAA34471.1; AAC14472.1; BAA04890.1; CAA92346.1; DAA12048.1 

Protein Name

 Transcriptional regulatory protein UME6 

Protein Synonyms/Alias

 Negative transcriptional regulator of IME2; Regulator of inducer of meiosis protein 16; Unscheduled meiotic gene expression protein 6 

Gene Name

 UME6 

Gene Synonyms/Alias

 CAR80; CARGR1; NIM2; RIM16; YDR207C; YD8142.04C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
345	PRNFYFNKDREITDP	acetylation	[1]
490	YIPPPPPKYINSKLD	acetylation	[1]
495	PPKYINSKLDGLRSR	acetylation	[1]
717	ATSPDFAKSKGKNVK	acetylation	[1]
736	SKAKQSSKKRPNNTT	acetylation	[2]
737	KAKQSSKKRPNNTTS	acetylation	[2]
745	RPNNTTSKSKANNSQ	acetylation	[2]
747	NNTTSKSKANNSQES	ubiquitination	[3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Gcn5p-dependent acetylation induces degradation of the meiotic transcriptional repressor Ume6p.
 Mallory MJ, Law MJ, Sterner DE, Berger SL, Strich R.
 Mol Biol Cell. 2012 May;23(9):1609-17. [PMID: 22438583]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Component of the RPD3C(L) histone deacetylase complex (HDAC) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Binds to the URS1 site (5'- AGCCGCCGA-3') and recruits the RPD3 histone deacetylase complex to the promoters to negatively regulate the expression of many genes including CAR1 (arginase), several required for sporulation, mating type switching, inositol metabolism, and oxidative carbon metabolism. Recruits also the ISW2 chromatin remodeling complex to promoters in a second gene repression pathway. Associates with the master regulator of meiosis IME1 in order to activate the expression of meiosis genes. Has both a positive and negative role in regulating phospholipid biosynthesis. 

Sequence Annotation

 DNA_BIND 771 798 Zn(2)-C6 fungal-type.
 REGION 508 594 SIN3-binding.
 MOD_RES 114 114 Phosphoserine.
 MOD_RES 141 141 Phosphoserine.
 MOD_RES 150 150 Phosphoserine.
 MOD_RES 228 228 Phosphoserine.
 MOD_RES 316 316 Phosphoserine.
 MOD_RES 318 318 Phosphoserine.
 MOD_RES 645 645 Phosphoserine.  

Keyword

 Activator; Chromatin regulator; Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 836 AA 

Protein Sequence

Gene Ontology

 GO:0033698; C:Rpd3L complex; IDA:SGD.
 GO:0070210; C:Rpd3L-Expanded complex; IDA:SGD.
 GO:0070491; F:repressing transcription factor binding; IDA:SGD.
 GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IDA:SGD.
 GO:0001078; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; IDA:SGD.
 GO:0000989; F:transcription factor binding transcription factor activity; IGI:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0034389; P:lipid particle organization; IMP:SGD.
 GO:1900471; P:negative regulation of inositol biosynthetic process by negative regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0010674; P:negative regulation of transcription from RNA polymerase II promoter during meiosis; IMP:SGD.
 GO:0007070; P:negative regulation of transcription from RNA polymerase II promoter during mitosis; IMP:SGD.
 GO:0001081; P:nitrogen catabolite repression of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:1900475; P:positive regulation of meiosis by negative regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:1900476; P:positive regulation of meiosis by positive regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:1900472; P:positive regulation of phosphatidylcholine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:1900525; P:positive regulation of phosphatidylserine biosynthetic process by positive regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0010673; P:positive regulation of transcription from RNA polymerase II promoter during meiosis; IMP:SGD.
 GO:0007124; P:pseudohyphal growth; IMP:SGD.
 GO:0009847; P:spore germination; IMP:SGD. 

Interpro

 IPR001138; Zn2-C6_fun-type_DNA-bd. 

Pfam

 PF00172; Zn_clus 

SMART

 SM00066; GAL4 

PROSITE

 PS00463; ZN2_CY6_FUNGAL_1
 PS50048; ZN2_CY6_FUNGAL_2 

PRINTS