UniProt Accession

 CYSJ_ECOLI; P38038; P14782; Q2MA65 

Genbank Protein ID

 M23008; U29579; U00096; AP009048 

Genbank Nucleotide ID

 AAA23650.1; AAA69274.1; AAC75806.1; BAE76841.1 

Protein Name

 Sulfite reductase [NADPH] flavoprotein alpha-component 

Protein Synonyms/Alias

 SiR-FP 

Gene Name

 cysJ 

Gene Synonyms/Alias

 b2764; JW2734 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
90	RDDLLAAKLNVKLVN	acetylation	[1]
94	LAAKLNVKLVNAGDY	acetylation	[1]
102	LVNAGDYKFKQIASE	acetylation	[1]
104	NAGDYKFKQIASEKL	acetylation	[1]
110	FKQIASEKLLIVVTS	acetylation	[1]
138	LHKFLFSKKAPKLEN	acetylation	[1]
139	HKFLFSKKAPKLENT	acetylation	[1]
171	SGKDFDSKLAELGGE	acetylation	[1]
206	ARVVDALKSRAPVAA	acetylation	[1]
233	IHTSPYSKDAPLVAS	acetylation	[1]
254	ITGRNSEKDVRHIEI	acetylation	[1]
297	LVELLWLKGDEPVTV	acetylation	[1]
348	LLPLVGDKAKLQHYA	acetylation	[1]
350	PLVGDKAKLQHYAAT	acetylation	[1]
507	VEWQRYVKDGVLTRI	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component. 

Sequence Annotation

 DOMAIN 64 202 Flavodoxin-like.
 DOMAIN 234 448 FAD-binding FR-type.
 NP_BIND 70 74 FMN.
 NP_BIND 117 122 FMN.
 NP_BIND 150 181 FMN.
 NP_BIND 386 389 FAD.
 NP_BIND 420 422 FAD.
 NP_BIND 519 527 NADP.
 BINDING 489 489 NADP.  

Keyword

 3D-structure; Amino-acid biosynthesis; Complete proteome; Cysteine biosynthesis; Direct protein sequencing; Electron transport; FAD; Flavoprotein; FMN; NADP; Oxidoreductase; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 599 AA 

Protein Sequence

Gene Ontology

 GO:0009337; C:sulfite reductase complex (NADPH); IDA:EcoCyc.
 GO:0050660; F:flavin adenine dinucleotide binding; IDA:EcoCyc.
 GO:0010181; F:FMN binding; IDA:EcoCyc.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0003958; F:NADPH-hemoprotein reductase activity; IBA:RefGenome.
 GO:0016709; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen; IBA:RefGenome.
 GO:0042602; F:riboflavin reductase (NADPH) activity; IDA:EcoCyc.
 GO:0004783; F:sulfite reductase (NADPH) activity; IEA:HAMAP.
 GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-KW.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0070814; P:hydrogen sulfide biosynthetic process; IEA:HAMAP.
 GO:0000103; P:sulfate assimilation; IEA:HAMAP. 

Interpro

 IPR010199; CysJ.
 IPR003097; FAD-binding_1.
 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001094; Flavdoxin.
 IPR008254; Flavodoxin/NO_synth.
 IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
 IPR023173; NADPH_Cyt_P450_Rdtase_dom3.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR017938; Riboflavin_synthase-like_b-brl. 

Pfam

 PF00667; FAD_binding_1
 PF00258; Flavodoxin_1
 PF00175; NAD_binding_1 

SMART

  

PROSITE

 PS51384; FAD_FR
 PS50902; FLAVODOXIN_LIKE 

PRINTS

 PR00369; FLAVODOXIN.
 PR00371; FPNCR.