UniProt Accession

 UBA5_HUMAN; Q9GZZ9; A6NJL3; D3DNC8; Q96ST1 

Genbank Protein ID

 AB154406; AY253672; AL136757; AK026904; AK027563; AC020632; CH471052; CH471052; CH471052; BC009737 

Genbank Nucleotide ID

 BAD15375.1; AAP79600.1; CAB66691.1; BAB15587.1; BAB55199.1; EAW79192.1; EAW79189.1; EAW79191.1; AAH09737.1 

Protein Name

 Ubiquitin-like modifier-activating enzyme 5 

Protein Synonyms/Alias

 Ubiquitin-activating enzyme 5; ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1 

Gene Name

 UBA5 

Gene Synonyms/Alias

 UBE1DC1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
42	GGRVRIEKMSSEVVD	ubiquitination	[1]
376	TVAYTIPKKQEDSVT	ubiquitination	[2]
377	VAYTIPKKQEDSVTE	ubiquitination	[2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 E1-like enzyme which activates UFM1 and SUMO2. 

Sequence Annotation

 ACT_SITE 250 250 Glycyl thioester intermediate.
 METAL 226 226 Zinc.
 METAL 229 229 Zinc.
 METAL 303 303 Zinc.
 METAL 308 308 Zinc.
 BINDING 83 83 ATP; via amide nitrogen.
 BINDING 104 104 ATP.
 BINDING 127 127 ATP.
 BINDING 150 150 ATP.
 BINDING 184 184 ATP.
 MOD_RES 45 45 Phosphoserine.
 MOD_RES 358 358 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation pathway; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 404 AA 

Protein Sequence

Gene Ontology

 GO:0016235; C:aggresome; IDA:HPA.
 GO:0005737; C:cytoplasm; IDA:LIFEdb.
 GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0048037; F:cofactor binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
 GO:0071566; F:UFM1 activating enzyme activity; IDA:UniProtKB.
 GO:0071569; P:protein ufmylation; IDA:UniProtKB. 

Interpro

 IPR006140; D-isomer_2_OHA_DH_NAD-bd.
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd. 

Pfam

 PF00899; ThiF 

SMART

  

PROSITE

  

PRINTS