UniProt Accession

 CNOT3_HUMAN; O75175; Q9NZN7; Q9UF76 

Genbank Protein ID

 AF180474; AB014591; AL133647; BC016474 

Genbank Nucleotide ID

 AAF29828.1; BAA31666.2; CAB63766.1; AAH16474.1 

Protein Name

 CCR4-NOT transcription complex subunit 3 

Protein Synonyms/Alias

 CCR4-associated factor 3; Leukocyte receptor cluster member 2 

Gene Name

 CNOT3 

Gene Synonyms/Alias

 KIAA0691; LENG2; NOT3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
737	EKWGQRKKEGFTFEY	ubiquitination	[1, 2, 3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of emryonic stem (ES) cell identity. 

Sequence Annotation

 REGION 661 753 Repressor domain.
 MOD_RES 292 292 Phosphothreonine.
 MOD_RES 299 299 Phosphoserine.  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Developmental protein; Nucleus; Phosphoprotein; Reference proteome; Repressor; RNA-mediated gene silencing; Transcription; Transcription regulation; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 753 AA 

Protein Sequence

Gene Ontology

 GO:0030014; C:CCR4-NOT complex; IDA:UniProtKB.
 GO:0000932; C:cytoplasmic mRNA processing body; ISS:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
 GO:2000036; P:regulation of stem cell maintenance; IMP:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR012270; CCR4-NOT_su3/5.
 IPR007282; NOT.
 IPR007207; Not_N. 

Pfam

 PF04153; NOT2_3_5
 PF04065; Not3 

SMART

  

PROSITE

  

PRINTS