CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023483
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sterol regulatory element-binding protein 1 
Protein Synonyms/Alias
 SREBP-1; Sterol regulatory element-binding transcription factor 1; Processed sterol regulatory element-binding protein 1 
Gene Name
 Srebf1 
Gene Synonyms/Alias
 Srebp1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
249SLLLTAVKTDAGATVubiquitination[1]
307HRLAAGSKALGSAQSubiquitination[1]
375QHSNQKLKQENLTLRubiquitination[1]
461AFEDSQVKAQRLPSHubiquitination[1]
576VHFWRHRKQADLDLAubiquitination[1]
911RAALYSFKAARALLDubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE- 1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC- 3'). Isoform SREBP-1A is much more active than isoform SREBP-1C in stimulating transcription from SRE-1-containing promoters. 
Sequence Annotation
 DOMAIN 317 367 bHLH.
 REGION 1 60 Transcriptional activation (acidic).
 REGION 227 487 Interaction with LMNA.
 REGION 367 388 Leucine-zipper.
 MOD_RES 108 108 Phosphotyrosine.
 MOD_RES 112 112 Phosphoserine.
 MOD_RES 115 115 Phosphoserine (By similarity).
 MOD_RES 331 331 Phosphoserine; by SIK1.
 MOD_RES 332 332 Phosphoserine; by SIK1.
 MOD_RES 389 389 Phosphoserine; by AMPK.
 MOD_RES 395 395 Phosphoserine; by SIK1.  
Keyword
 Activator; Alternative splicing; Cholesterol metabolism; Complete proteome; Cytoplasmic vesicle; DNA-binding; Endoplasmic reticulum; Golgi apparatus; Lipid metabolism; Membrane; Nucleus; Phosphoprotein; Reference proteome; Steroid metabolism; Sterol metabolism; Transcription; Transcription regulation; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1134 AA 
Protein Sequence
MDELAFGEAA LEQTLAEMCE LDTAVLNDIE DMLQLINNQD SDFPGLFDAP YAGGETGDTG 60
PSSPGANSPE SFSSASLASS LEAFLGGPKV TPAPLSPPPS APAALKMYPS VSPFSPGPGI 120
KEEPVPLTIL QPAAPQPSPG TLLPPSFPAP PVQLSPAPVL GYSSLPSGFS GTLPGNTQQP 180
PSSLPLAPAP GVLPTPALHT QVQSLASQQP LPASAAPRTN TVTSQVQQVP VVLQPHFIKA 240
DSLLLTAVKT DAGATVKTAG ISTLAPGTAV QAGPLQTLVS GGTILATVPL VVDTDKLPIH 300
RLAAGSKALG SAQSRGEKRT AHNAIEKRYR SSINDKIVEL KDLVVGTEAK LNKSAVLRKA 360
IDYIRFLQHS NQKLKQENLT LRSAHKSKSL KDLVSACGSG GGTDVSMEGM KPEVVETLTP 420
PPSDAGSPSQ SSPLSFGSRA SSSGGSDSEP DSPAFEDSQV KAQRLPSHSR GMLDRSRLAL 480
CVLAFLCLTC NPLASLFGWG ILTPSDATGT HRSSGRSMLE AESRDGSNWT QWLLPPLVWL 540
ANGLLVLACL ALLFVYGEPV TRPHSGPAVH FWRHRKQADL DLARGDFPQA AQQLWLALQA 600
LGRPLPTSNL DLACSLLWNL IRHLLQRLWV GRWLAGQAGG LLRDRGLRKD ARASARDAAV 660
VYHKLHQLHA MGKYTGGHLA ASNLALSALN LAECAGDAIS MATLAEIYVA AALRVKTSLP 720
RALHFLTRFF LSSARQACLA QSGSVPLAMQ WLCHPVGHRF FVDGDWAVHG APPESLYSVA 780
GNPVDPLAQV TRLFREHLLE RALNCIAQPS PGAADGDREF SDALGYLQLL NSCSDAAGAP 840
ACSFSVSSSM AATTGPDPVA KWWASLTAVV IHWLRRDEEA AERLYPLVEH IPQVLQDTER 900
PLPRAALYSF KAARALLDHR KVESSPASLA ICEKASGYLR DSLASTPTGS SIDKAMQLLL 960
CDLLLVARTS LWQRQQSPAS VQVAHGTSNG PQASALELRG FQHDLSSLRR LAQSFRPAMR 1020
RVFLHEATAR LMAGASPART HQLLDRSLRR RAGSSGKGGT TAELEPRPTW REHTEALLLA 1080
SCYLPPAFLS APGQRMSMLA EAARTVEKLG DHRLLLDCQQ MLLRLGGGTT VTSS 1134 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0012507; C:ER to Golgi transport vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; ISS:HGNC.
 GO:0032810; F:sterol response element binding; ISS:HGNC.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:MGI.
 GO:0007568; P:aging; IEA:Compara.
 GO:0009267; P:cellular response to starvation; IDA:HGNC.
 GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW.
 GO:0008286; P:insulin receptor signaling pathway; IDA:MGI.
 GO:0008610; P:lipid biosynthetic process; IDA:UniProtKB.
 GO:0030324; P:lung development; IEA:Compara.
 GO:0046676; P:negative regulation of insulin secretion; IMP:MGI.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0045542; P:positive regulation of cholesterol biosynthetic process; IMP:MGI.
 GO:0045723; P:positive regulation of fatty acid biosynthetic process; TAS:BHF-UCL.
 GO:0031065; P:positive regulation of histone deacetylation; IDA:MGI.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:BHF-UCL.
 GO:0010867; P:positive regulation of triglyceride biosynthetic process; IDA:UniProtKB.
 GO:0003062; P:regulation of heart rate by chemical signal; IMP:MGI.
 GO:0051591; P:response to cAMP; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0070542; P:response to fatty acid; IEA:Compara.
 GO:0032094; P:response to food; IEA:Compara.
 GO:0033762; P:response to glucagon stimulus; IEA:Compara.
 GO:0009749; P:response to glucose stimulus; IMP:MGI.
 GO:0032570; P:response to progesterone stimulus; IEA:Compara.
 GO:0032526; P:response to retinoic acid; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR011598; bHLH_dom. 
Pfam
 PF00010; HLH 
SMART
 SM00353; HLH 
PROSITE
 PS50888; BHLH 
PRINTS