CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043751
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 S-formylglutathione hydrolase 
Protein Synonyms/Alias
  
Gene Name
 ESD 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
115LKNPGKYKSVSAFAPubiquitination[1, 2, 3]
133PVLCPWGKKAFSGYLubiquitination[3, 4]
134VLCPWGKKAFSGYLGubiquitination[3, 5]
146YLGTDQSKWKAYDATubiquitination[1, 2, 3, 5]
148GTDQSKWKAYDATHLacetylation[5, 6, 7]
148GTDQSKWKAYDATHLubiquitination[1, 3, 4, 5]
157YDATHLVKSYPGSQLubiquitination[1]
195IAACTEKKIPVVFRLubiquitination[3, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [7] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 230 AA 
Protein Sequence
XLTCTEQNFI SKSGYHQSAS EHGLVVIAPD TSPRGCNIKG EDESWDFGTG AGFYVDATED 60
PWKTNYRMYS YVTEELPQLI NANFPVDPQR MSIFGHSMGG HGALICALKN PGKYKSVSAF 120
APICNPVLCP WGKKAFSGYL GTDQSKWKAY DATHLVKSYP GSQLDILIDQ GKDDQFLLDG 180
QLLPDNFIAA CTEKKIPVVF RLQEHGRVKS GLHSLKRISL RFVVIESTTE 230 
Gene Ontology
 GO:0004091; F:carboxylesterase activity; IEA:InterPro.
 GO:0018738; F:S-formylglutathione hydrolase activity; IEA:InterPro.
 GO:0046294; P:formaldehyde catabolic process; IEA:InterPro. 
Interpro
 IPR000801; Esterase_put.
 IPR014186; S-formylglutathione_hydrol. 
Pfam
 PF00756; Esterase 
SMART
  
PROSITE
  
PRINTS