CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006932
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Trehalose synthase complex regulatory subunit TSL1 
Protein Synonyms/Alias
 Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 123 kDa subunit 
Gene Name
 TSL1 
Gene Synonyms/Alias
 YML100W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
263KQRPLLAKQPSNLKYubiquitination[1]
452AFEDHSWKFYRNLNQacetylation[2]
913VAKILEDKVERLPGSubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Regulatory subunit of the trehalose synthase complex that catalyzes the production of trehalose from glucose-6- phosphate and UDP-glucose in a two step process. May stabilize the trehalose synthase complex, and confer sensitivity to physiological concentrations of phosphate and to fructose 6- phosphate. 
Sequence Annotation
 REPEAT 144 150 1.
 REPEAT 158 164 2.
 REGION 144 164 2 X 7 AA repeats of R-I-A-S-P-I-Q.
 REGION 320 812 TPS complex domain.
 MOD_RES 49 49 Phosphoserine.
 MOD_RES 53 53 Phosphoserine.
 MOD_RES 56 56 Phosphoserine.
 MOD_RES 71 71 Phosphoserine.
 MOD_RES 77 77 Phosphoserine.
 MOD_RES 135 135 Phosphoserine.
 MOD_RES 147 147 Phosphoserine.
 MOD_RES 161 161 Phosphoserine.
 MOD_RES 229 229 Phosphoserine.
 MOD_RES 303 303 Phosphoserine.
 MOD_RES 815 815 Phosphothreonine.  
Keyword
 Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1098 AA 
Protein Sequence
MALIVASLFL PYQPQFELDT SLPENSQVDS SLVNIQAMAN DQQQQRALSN NISQESLVAP 60
APEQGVPPAI SRSATRSPSA FNRASSTTNT ATLDDLVSSD IFMENLTANA TTSHTPTSKT 120
MLKPRKNGSV ERFFSPSSNI PTDRIASPIQ HEHDSGSRIA SPIQQQQQDP TTNLLKNVNK 180
SLLVHSLLNN TSQTSLEGPN NHIVTPKSRA GNRPTSAATS LVNRTKQGSA SSGSSGSSAP 240
PSIKRITPHL TASAAKQRPL LAKQPSNLKY SELADISSSE TSSQHNESDP DDLTTAPDEE 300
YVSDLEMDDA KQDYKVPKFG GYSNKSKLKK YALLRSSQEL FSRLPWSIVP SIKGNGAMKN 360
AINTAVLENI IPHRHVKWVG TVGIPTDEIP ENILANISDS LKDKYDSYPV LTDDDTFKAA 420
YKNYCKQILW PTLHYQIPDN PNSKAFEDHS WKFYRNLNQR FADAIVKIYK KGDTIWIHDY 480
HLMLVPQMVR DVLPFAKIGF TLHVSFPSSE VFRCLAQREK ILEGLTGADF VGFQTREYAR 540
HFLQTSNRLL MADVVHDEEL KYNGRVVSVR FTPVGIDAFD LQSQLKDGSV MQWRQLIRER 600
WQGKKLIVCR DQFDRIRGIH KKLLAYEKFL VENPEYVEKS TLIQICIGSS KDVELERQIM 660
IVVDRINSLS TNISISQPVV FLHQDLDFSQ YLALSSEADL FVVSSLREGM NLTCHEFIVC 720
SEDKNAPLLL SEFTGSASLL NDGAIIINPW DTKNFSQAIL KGLEMPFDKR RPQWKKLMKD 780
IINNDSTNWI KTSLQDIHIS WQFNQEGSKI FKLNTKTLME DYQSSKKRMF VFNIAEPPSS 840
RMISILNDMT SKGNIVYIMN SFPKPILENL YSRVQNIGLI AENGAYVSLN GVWYNIVDQV 900
DWRNDVAKIL EDKVERLPGS YYKINESMIK FHTENAEDQD RVASVIGDAI THINTVFDHR 960
GIHAYVYKNV VSVQQVGLSL SAAQFLFRFY NSASDPLDTS SGQITNIQTP SQQNPSDQEQ 1020
QPPASPTVSM NHIDFACVSG SSSPVLEPLF KLVNDEASEG QVKAGHAIVY GDATSTYAKE 1080
HVNGLNELFT IISRIIED 1098 
Gene Ontology
 GO:0005946; C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming); IMP:SGD.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0030234; F:enzyme regulator activity; IDA:SGD.
 GO:0005992; P:trehalose biosynthetic process; IMP:SGD. 
Interpro
 IPR001830; Glyco_trans_20.
 IPR023214; HAD-like_dom.
 IPR003337; Trehalose_PPase. 
Pfam
 PF00982; Glyco_transf_20
 PF02358; Trehalose_PPase 
SMART
  
PROSITE
  
PRINTS