UniProt Accession

 PRP19_HUMAN; Q9UMS4 

Genbank Protein ID

 AJ131186; BC008719; BC018665; BC018698 

Genbank Nucleotide ID

 CAB51857.1; AAH08719.1; AAH18665.1; AAH18698.1 

Protein Name

 Pre-mRNA-processing factor 19 

Protein Synonyms/Alias

 Nuclear matrix protein 200; PRP19/PSO4 homolog; hPso4; Senescence evasion factor 

Gene Name

 PRPF19 

Gene Synonyms/Alias

 NMP200; PRP19; SNEV 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
122	RVIARLTKEVTAARE	acetylation	[1]
122	RVIARLTKEVTAARE	ubiquitination	[2, 3]
179	IIQKLQDKATVLTTE	ubiquitination	[3]
200	TVPEELVKPEELSKY	ubiquitination	[3]
206	VKPEELSKYRQVASH	ubiquitination	[3]
244	ILTGGADKNVVVFDK	acetylation	[4]
244	ILTGGADKNVVVFDK	ubiquitination	[3, 4, 5, 6]
261	EQILATLKGHTKKVT	acetylation	[1]
261	EQILATLKGHTKKVT	ubiquitination	[3, 4]
265	ATLKGHTKKVTSVVF	ubiquitination	[3]
266	TLKGHTKKVTSVVFH	ubiquitination	[7, 8]
380	QIKIWDLKERTNVAN	ubiquitination	[3, 4, 5]
428	LRKLKNFKTLQLDNN	ubiquitination	[7, 8]
500	TGMDRSLKFYSL***	ubiquitination	[3]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. 

Sequence Annotation

 DOMAIN 2 68 U-box.
 REPEAT 219 259 WD 1.
 REPEAT 262 301 WD 2.
 REPEAT 304 345 WD 3.
 REPEAT 348 387 WD 4.
 REPEAT 390 429 WD 5.
 REPEAT 433 472 WD 6.
 REPEAT 473 503 WD 7.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 122 122 N6-acetyllysine.
 MOD_RES 261 261 N6-acetyllysine.  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; DNA damage; DNA repair; DNA-binding; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat; Spliceosome; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 504 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0005811; C:lipid particle; IEA:Compara.
 GO:0016607; C:nuclear speck; IDA:BHF-UCL.
 GO:0000974; C:Prp19 complex; IDA:UniProtKB.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0000151; C:ubiquitin ligase complex; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0034450; F:ubiquitin-ubiquitin ligase activity; IDA:MGI.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0001833; P:inner cell mass cell proliferation; IEA:Compara.
 GO:0008610; P:lipid biosynthetic process; IEA:Compara.
 GO:0045665; P:negative regulation of neuron differentiation; IEA:Compara.
 GO:0048711; P:positive regulation of astrocyte differentiation; IEA:Compara.
 GO:0048026; P:positive regulation of mRNA splicing, via spliceosome; IEA:Compara.
 GO:0000209; P:protein polyubiquitination; IDA:MGI.
 GO:0000245; P:spliceosomal complex assembly; IMP:BHF-UCL. 

Interpro

 IPR020472; G-protein_beta_WD-40_rep.
 IPR013915; Pre-mRNA_splic_Prp19.
 IPR000772; Ricin_B_lectin.
 IPR003613; Ubox_domain.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom.
 IPR013083; Znf_RING/FYVE/PHD. 

Pfam

 PF08606; Prp19
 PF04564; U-box
 PF00400; WD40 

SMART

 SM00504; Ubox
 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS

 PR00320; GPROTEINBRPT.