UniProt Accession

 PCP_HUMAN; P42785; A8MU24; B2R7B7; B3KRK5; B5BU34 

Genbank Protein ID

 L13977; AK091786; AK312919; AB451270; AB451397; AP000893; AP001646; CH471076; CH471076; BC001500 

Genbank Nucleotide ID

 AAA99891.1; BAG52417.1; BAG35764.1; BAG70084.1; BAG70211.1; EAW75074.1; EAW75075.1; AAH01500.1 

Protein Name

 Lysosomal Pro-X carboxypeptidase 

Protein Synonyms/Alias

 Angiotensinase C; Lysosomal carboxypeptidase C; Proline carboxypeptidase; Prolylcarboxypeptidase; PRCP 

Gene Name

 PRCP 

Gene Synonyms/Alias

 PCP 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
66	HFGFNTVKTFNQRYL	ubiquitination	[1]
77	QRYLVADKYWKKNGG	ubiquitination	[2]
137	PFGDNSFKDSRHLNF	ubiquitination	[3]
462	HHLDLRTKNALDPMS	ubiquitination	[3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. 

Sequence Annotation

 REGION 194 334 SKS domain.
 ACT_SITE 179 179 Charge relay system.
 ACT_SITE 430 430 Charge relay system.
 ACT_SITE 455 455 Charge relay system.
 CARBOHYD 47 47 N-linked (GlcNAc...) (Potential).
 CARBOHYD 101 101 N-linked (GlcNAc...).
 CARBOHYD 317 317 N-linked (GlcNAc...).
 CARBOHYD 336 336 N-linked (GlcNAc...).
 CARBOHYD 345 345 N-linked (GlcNAc...).
 CARBOHYD 415 415 N-linked (GlcNAc...).
 DISULFID 215 372
 DISULFID 233 310
 DISULFID 264 343
 DISULFID 364 394  

Keyword

 3D-structure; Alternative splicing; Carboxypeptidase; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Hydrolase; Lysosome; Polymorphism; Protease; Reference proteome; Signal; Zymogen. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 496 AA 

Protein Sequence

Gene Ontology

 GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0004185; F:serine-type carboxypeptidase activity; TAS:Reactome.
 GO:0007597; P:blood coagulation, intrinsic pathway; TAS:Reactome.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR008758; Peptidase_S28. 

Pfam

 PF05577; Peptidase_S28 

SMART

  

PROSITE

  

PRINTS