CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-026042
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Ubiquitin carboxyl-terminal hydrolase isozyme L5 
Protein Synonyms/Alias
  
Gene Name
 UCHL5 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
45LEPENFEKLKPVHGLubiquitination[1]
47PENFEKLKPVHGLIFubiquitination[2]
154QMFEFDTKTSAKEEDubiquitination[3]
206AVRPVIEKRIQKDGFubiquitination[3]
210VIEKRIQKDGFSPCCubiquitination[2, 4]
227WSQTPELKPSACLDLubiquitination[2, 3, 4, 5]
261RKMIYEQKIAELQRQubiquitination[3]
341QLIPLVEKAKEKQNAubiquitination[1, 2, 3, 4, 5, 6, 7]
343IPLVEKAKEKQNAKKubiquitination[2, 5]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 355 AA 
Protein Sequence
MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP 60
GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS 120
FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG 180
LREGPIDLGA CNQDDWISAV RPVIEKRIQK DGFSPCCPGW SQTPELKPSA CLDLPKWYSE 240
GEIRFNLMAI VSDRKMIYEQ KIAELQRQLA EEPMDTDQGN SMLSAIQSEV AKNQMLIEEE 300
VQKLKRYKIE NIRRKHNYLP FIMELLKTLA EHQQLIPLVE KAKEKQNAKK AQETK 355 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0008242; F:omega peptidase activity; IEA:InterPro.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR001578; Peptidase_C12.
 IPR017390; Ubiquitinyl_hydrolase_UCH37. 
Pfam
 PF01088; Peptidase_C12 
SMART
  
PROSITE
  
PRINTS
 PR00707; UBCTHYDRLASE.