CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006691
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sterol regulatory element-binding protein 1 
Protein Synonyms/Alias
 SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1; Processed sterol regulatory element-binding protein 1 
Gene Name
 SREBF1 
Gene Synonyms/Alias
 BHLHD1; SREBP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
286SLLLTAMKTDGATVKubiquitination[1]
343NRLAAGSKAPASAQSubiquitination[2]
377NDKIIELKDLVVGTEubiquitination[1, 2]
386LVVGTEAKLNKSAVLubiquitination[1, 2]
389GTEAKLNKSAVLRKAubiquitination[2]
395NKSAVLRKAIDYIRFubiquitination[2]
409FLQHSNQKLKQENLSubiquitination[2]
411QHSNQKLKQENLSLRubiquitination[2]
425RTAVHKSKSLKDLVSubiquitination[2]
428VHKSKSLKDLVSACGubiquitination[2]
500VFEDSKAKPEQRPSLubiquitination[2]
617VYFWRHRKQADLDLAubiquitination[1, 3]
705DAALVYHKLHQLHTMubiquitination[1, 2]
757VAAALRVKTSLPRALubiquitination[2]
954RAALHSFKAARALLGubiquitination[1, 2, 3]
964RALLGCAKAESGPASubiquitination[2, 3]
977ASLTICEKASGYLQDubiquitination[2]
1100RRAGPGGKGGAVAELubiquitination[1, 2, 3]
1151EAARTLEKLGDRRLLubiquitination[2, 4]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). 
Sequence Annotation
 DOMAIN 323 373 bHLH.
 REGION 1 60 Transcriptional activation (acidic).
 REGION 234 497 Interaction with LMNA (By similarity).
 REGION 373 394 Leucine-zipper.
 MOD_RES 110 110 Phosphotyrosine (By similarity).
 MOD_RES 117 117 Phosphoserine.
 MOD_RES 337 337 Phosphoserine; by SIK1 (By similarity).
 MOD_RES 338 338 Phosphoserine; by SIK1 (By similarity).
 MOD_RES 396 396 Phosphoserine; by AMPK (By similarity).
 MOD_RES 402 402 Phosphoserine; by SIK1 (By similarity).  
Keyword
 3D-structure; Activator; Alternative splicing; Cholesterol metabolism; Complete proteome; Cytoplasmic vesicle; Direct protein sequencing; DNA-binding; Endoplasmic reticulum; Golgi apparatus; Lipid metabolism; Membrane; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Steroid metabolism; Sterol metabolism; Transcription; Transcription regulation; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1147 AA 
Protein Sequence
MDEPPFSEAA LEQALGEPCD LDAALLTDIE GEVGAGRGRA NGLDAPRAGA DRGAMDCTFE 60
DMLQLINNQD SDFPGLFDPP YAGSGAGGTD PASPDTSSPG SLSPPPATLS SSLEAFLSGP 120
QAAPSPLSPP QPAPTPLKMY PSMPAFSPGP GIKEESVPLS ILQTPTPQPL PGALLPQSFP 180
APAPPQFSST PVLGYPSPPG GFSTGSPPGN TQQPLPGLPL ASPPGVPPVS LHTQVQSVVP 240
QQLLTVTAAP TAAPVTTTVT SQIQQVPVLL QPHFIKADSL LLTAMKTDGA TVKAAGLSPL 300
VSGTTVQTGP LPTLVSGGTI LATVPLVVDA EKLPINRLAA GSKAPASAQS RGEKRTAHNA 360
IEKRYRSSIN DKIIELKDLV VGTEAKLNKS AVLRKAIDYI RFLQHSNQKL KQENLSLRTA 420
VHKSKSLKDL VSACGSGGNT DVLMEGVKTE VEDTLTPPPS DAGSPFQSSP LSLGSRGSGS 480
GGSGSDSEPD SPVFEDSKAK PEQRPSLHSR GMLDRSRLAL CTLVFLCLSC NPLASLLGAR 540
GLPSPSDTTS VYHSPGRNVL GTESRDGPGW AQWLLPPVVW LLNGLLVLVS LVLLFVYGEP 600
VTRPHSGPAV YFWRHRKQAD LDLARGDFAQ AAQQLWLALR ALGRPLPTSH LDLACSLLWN 660
LIRHLLQRLW VGRWLAGRAG GLQQDCALRV DASASARDAA LVYHKLHQLH TMGKHTGGHL 720
TATNLALSAL NLAECAGDAV SVATLAEIYV AAALRVKTSL PRALHFLTRF FLSSARQACL 780
AQSGSVPPAM QWLCHPVGHR FFVDGDWSVL STPWESLYSL AGNPVDPLAQ VTQLFREHLL 840
ERALNCVTQP NPSPGSADGD KEFSDALGYL QLLNSCSDAA GAPAYSFSIS SSMATTTGVD 900
PVAKWWASLT AVVIHWLRRD EEAAERLCPL VEHLPRVLQE SERPLPRAAL HSFKAARALL 960
GCAKAESGPA SLTICEKASG YLQDSLATTP ASSSIDKAVQ LFLCDLLLVV RTSLWRQQQP 1020
PAPAPAAQGT SSRPQASALE LRGFQRDLSS LRRLAQSFRP AMRRVFLHEA TARLMAGASP 1080
TRTHQLLDRS LRRRAGPGGK GGAVAELEPR PTRREHAEAL LLASCYLPPG FLSAPGQRVG 1140
MLAEAARTLE KLGDRRLLHD CQQMLMRLGG GTTVTSS 1177 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0012507; C:ER to Golgi transport vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005635; C:nuclear envelope; TAS:ProtInc.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:HGNC.
 GO:0032810; F:sterol response element binding; IDA:HGNC.
 GO:0007568; P:aging; IEA:Compara.
 GO:0044255; P:cellular lipid metabolic process; TAS:Reactome.
 GO:0009267; P:cellular response to starvation; ISS:HGNC.
 GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW.
 GO:0008286; P:insulin receptor signaling pathway; IEA:Compara.
 GO:0008610; P:lipid biosynthetic process; ISS:UniProtKB.
 GO:0030324; P:lung development; IEA:Compara.
 GO:0046676; P:negative regulation of insulin secretion; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045542; P:positive regulation of cholesterol biosynthetic process; IEA:Compara.
 GO:0031065; P:positive regulation of histone deacetylation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0010867; P:positive regulation of triglyceride biosynthetic process; ISS:UniProtKB.
 GO:0019217; P:regulation of fatty acid metabolic process; IEA:Compara.
 GO:0003062; P:regulation of heart rate by chemical signal; IEA:Compara.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0051591; P:response to cAMP; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0070542; P:response to fatty acid; IEA:Compara.
 GO:0032094; P:response to food; IEA:Compara.
 GO:0033762; P:response to glucagon stimulus; IEA:Compara.
 GO:0009749; P:response to glucose stimulus; IEA:Compara.
 GO:0032570; P:response to progesterone stimulus; IEA:Compara.
 GO:0032526; P:response to retinoic acid; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0032933; P:SREBP signaling pathway; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR011598; bHLH_dom. 
Pfam
 PF00010; HLH 
SMART
 SM00353; HLH 
PROSITE
 PS50888; BHLH 
PRINTS