UniProt Accession

 NAGA_HUMAN; Q9Y303; B4DL77; Q8WV54 

Genbank Protein ID

 AF132948; AK296877; CH471112; BC018734 

Genbank Nucleotide ID

 AAD27723.1; BAG59439.1; EAW85500.1; AAH18734.1 

Protein Name

 Putative N-acetylglucosamine-6-phosphate deacetylase 

Protein Synonyms/Alias

 Amidohydrolase domain-containing protein 2; GlcNAc 6-P deacetylase 

Gene Name

 AMDHD2 

Gene Synonyms/Alias

 CGI-14 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
25	CRILRGGKLLREDLW	ubiquitination	[1]
43	GRILDPEKLFFEERR	ubiquitination	[1, 2]
120	SPPEVYHKVVPQIPV	ubiquitination	[1]
128	VVPQIPVKSGGPHGA	ubiquitination	[1]
326	TAYVAGTKTLSGSIA	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Hydrolyzes the N-glycolyl group from N- glycolylglucosamine 6-phosphate (GlcNGc-6-P) in the N- glycolylneuraminic acid (Neu5Gc) degradation pathway. Although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded. 

Sequence Annotation

 REGION 235 236 Substrate binding (By similarity).
 REGION 328 330 Substrate binding (By similarity).
 ACT_SITE 294 294 Proton donor/acceptor (By similarity).
 METAL 143 143 Divalent metal cation (By similarity).
 METAL 211 211 Divalent metal cation; via tele nitrogen
 METAL 232 232 Divalent metal cation; via tele nitrogen
 BINDING 154 154 Substrate; via amide nitrogen (By
 BINDING 243 243 Substrate (By similarity).
 BINDING 272 272 Substrate; via tele nitrogen (By  

Keyword

 Alternative splicing; Carbohydrate metabolism; Complete proteome; Hydrolase; Metal-binding; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 409 AA 

Protein Sequence

Gene Ontology

 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008448; F:N-acetylglucosamine-6-phosphate deacetylase activity; IDA:UniProtKB.
 GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
 GO:0006044; P:N-acetylglucosamine metabolic process; IEA:InterPro.
 GO:0019262; P:N-acetylneuraminate catabolic process; TAS:UniProtKB. 

Interpro

 IPR006680; Amidohydro_1.
 IPR003764; GlcNAc_6-P_deAcase.
 IPR011059; Metal-dep_hydrolase_composite. 

Pfam

 PF01979; Amidohydro_1 

SMART

  

PROSITE

  

PRINTS