CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019122
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 FAS-associated factor 2 
Protein Synonyms/Alias
 Protein ETEA; UBX domain-containing protein 3B; UBX domain-containing protein 8 
Gene Name
 FAF2 
Gene Synonyms/Alias
 ETEA; KIAA0887; UBXD8; UBXN3B 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
16LTQEQTEKLLQFQDLubiquitination[1]
146FMHSFEEKYGRAHPVubiquitination[1, 2, 3, 4]
167SQALNDAKRELRFLLacetylation[5]
167SQALNDAKRELRFLLubiquitination[1, 2, 4, 6, 7, 8, 9, 10, 11, 12]
215FWACSTNKPEGYRVSubiquitination[4, 12]
239FLAMIMLKDRRMTVVubiquitination[1]
307SLRADQEKERKKREEubiquitination[1, 4]
321ERERKRRKEEEVQQQubiquitination[1, 2]
329EEEVQQQKLAEERRRubiquitination[1, 2, 3, 4, 7, 12]
343RQNLQEEKERKLECLubiquitination[2, 12]
367ESVKIIFKLPNDSRVubiquitination[1, 2, 4, 6, 10, 11, 12, 13]
400SLKESPEKFQIEANFubiquitination[1, 2, 4, 6, 8, 10, 11, 12]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [11] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [12] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [13] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048
Functional Description
 May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. 
Sequence Annotation
 DOMAIN 12 48 UBA.
 DOMAIN 357 439 UBX.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 167 167 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Coiled coil; Complete proteome; Cytoplasm; Endoplasmic reticulum; Lipid droplet; Reference proteome; Unfolded protein response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 445 AA 
Protein Sequence
MAAPEERDLT QEQTEKLLQF QDLTGIESMD QCRHTLEQHN WNIEAAVQDR LNEQEGVPSV 60
FNPPPSRPLQ VNTADHRIYS YVVSRPQPRG LLGWGYYLIM LPFRFTYYTI LDIFRFALRF 120
IRPDPRSRVT DPVGDIVSFM HSFEEKYGRA HPVFYQGTYS QALNDAKREL RFLLVYLHGD 180
DHQDSDEFCR NTLCAPEVIS LINTRMLFWA CSTNKPEGYR VSQALRENTY PFLAMIMLKD 240
RRMTVVGRLE GLIQPDDLIN QLTFIMDANQ TYLVSERLER EERNQTQVLR QQQDEAYLAS 300
LRADQEKERK KREERERKRR KEEEVQQQKL AEERRRQNLQ EEKERKLECL PPEPSPDDPE 360
SVKIIFKLPN DSRVERRFHF SQSLTVIHDF LFSLKESPEK FQIEANFPRR VLPCIPSEEW 420
PNPPTLQEAG LSHTEVLFVQ DLTDE 445 
Gene Ontology
 GO:0034098; C:Cdc48p-Npl4p-Ufd1p AAA ATPase complex; IDA:BHF-UCL.
 GO:0005811; C:lipid particle; IDA:UniProtKB.
 GO:0043130; F:ubiquitin binding; IDA:BHF-UCL.
 GO:0031625; F:ubiquitin protein ligase binding; IDA:BHF-UCL.
 GO:0006986; P:response to unfolded protein; IEA:UniProtKB-KW. 
Interpro
 IPR006577; UAS.
 IPR009060; UBA-like.
 IPR001012; UBX. 
Pfam
 PF00789; UBX 
SMART
 SM00594; UAS 
PROSITE
 PS50030; UBA
 PS50033; UBX 
PRINTS