CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005417
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bud site selection protein 3 
Protein Synonyms/Alias
  
Gene Name
 BUD3 
Gene Synonyms/Alias
 YCL014W; YCL012W; YCL013W; YCL12W; YCL13W; YCL14W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
792RSYTSDLKDSPSGDNubiquitination[1, 2]
963SSVVRDTKYVSYNPSubiquitination[1, 2]
981TENTSNEKNVEPKADubiquitination[2]
1035SSSVHGNKELPDLAEubiquitination[2]
1104IPKHYRNKQENINSSubiquitination[2]
1124EGKVPQEKGVSNENTubiquitination[2]
1164TENSNELKDTNNEGKubiquitination[2]
1185LGDDYSDKETAKEITubiquitination[2]
1275EDAAIINKSQDDAKAubiquitination[2]
1503SSDLSSNKTVLGNAQubiquitination[2]
Reference
 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Co-assembles with BUD4 at bud sites. BUD4 and BUD3 may cooperate to recognize a spatial landmark (the neck filaments) during mitosis and they subsequently become a landmark for establishing the axial budding pattern in G1. 
Sequence Annotation
 MOD_RES 766 766 Phosphoserine.
 MOD_RES 1045 1045 Phosphothreonine.
 MOD_RES 1063 1063 Phosphoserine.
 MOD_RES 1075 1075 Phosphoserine.
 MOD_RES 1134 1134 Phosphoserine.
 MOD_RES 1149 1149 Phosphoserine.
 MOD_RES 1157 1157 Phosphothreonine.
 MOD_RES 1160 1160 Phosphoserine.
 MOD_RES 1228 1228 Phosphoserine.
 MOD_RES 1254 1254 Phosphoserine.
 MOD_RES 1257 1257 Phosphoserine.
 MOD_RES 1390 1390 Phosphoserine.
 MOD_RES 1412 1412 Phosphoserine.
 MOD_RES 1429 1429 Phosphothreonine.
 MOD_RES 1440 1440 Phosphothreonine.
 MOD_RES 1443 1443 Phosphoserine.
 MOD_RES 1501 1501 Phosphoserine.
 MOD_RES 1549 1549 Phosphoserine.
 MOD_RES 1589 1589 Phosphoserine.
 MOD_RES 1614 1614 Phosphoserine.  
Keyword
 Cell cycle; Complete proteome; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1636 AA 
Protein Sequence
MEKDLSSLYS EKKDKENDET LFNIKLSKSV VETTPLNGHS LFDDDKSLSD WTDNVFTQSV 60
FYHGSDDLIW GKFFVCVYKS PNSNKLNAII FDKLGTSCFE SVDISSNSQY YPAIENLSPS 120
DQESNVKKCI AVILLQRYPL LSPSDLSQIL SNKSENCDYD PPYAGDLASS CQLITAVPPE 180
DLGKRFFTSG LLQNRFVSST LLDVIYENNE STIELNNRLV FHLGEQLEQL FNPVTEYSPE 240
QTEYGYKAPE DELPTESDDD LVKAICNELL QLQTNFTFNL VEFLQKFLIA LRVRVLNEEI 300
NGLSTTKLNR LFPPTIDEVT RINCIFLDSL KTAIPYGSLE VLKACSITIP YFYKAYTRHE 360
AATKNFSKDI KLFIRHFSNV IPEREVYTEM KIESIIKGPQ EKLLKLKLII ERLWKSKKWR 420
PKNQEMAKKC YNNIIDVIDS FGKLDSPLHS YSTRVFTPSG KILTELAKCW PVELQYKWLK 480
RRVVGVYDVV DLNDENKRNL LVIFSDYVVF INILEAESYY TSDGSNRPLI SDILMNSLIN 540
EVPLPSKIPK LKVERHCYID EVLVSILDKS TLRFDRLKGK DSFSMVCKLS SAFISSSSVA 600
DLITKARILE KDTAFHLFKA SRSHFTLYST AHELCAYDSE KIKSKFALFL NIPPSKEILE 660
VNNLHLAFFA RFCSNDGRDN IVILDVLTKH DDKHIEVTSD NIVFTIINQL AIEIPICFSS 720
LNSSMAKDLL CVNENLIKNL EHQLEEVKHP STDEHRAVNS KLSGASDFDA THEKKRSYGT 780
ITTFRSYTSD LKDSPSGDNS NVTKETKEIL PVKPTKKSSK KPREIQKKTK TNASKAEHIE 840
KKKPNKGKGF FGVLKNVFGS KSKSKPSPVQ RVPKKISQRH PKSPVKKPMT SEKKSSPKRA 900
VVSSPKIKKK STSFSTKESQ TAKSSLRAVE FKSDDLIGKP PDVGNGAHPQ ENTRISSVVR 960
DTKYVSYNPS QPVTENTSNE KNVEPKADQS TKQDNISNFA DVEVSASSYP EKLDAETDDQ 1020
IIGKATNSSS VHGNKELPDL AEVTTANRVS TTSAGDQRID TQSEFLRAAD VENLSDDDEH 1080
RQNESRVFND DLFGDFIPKH YRNKQENINS SSNLFPEGKV PQEKGVSNEN TNISLKTNED 1140
ASTLTQKLSP QASKVLTENS NELKDTNNEG KDAKDIKLGD DYSDKETAKE ITKPKNFVEG 1200
ITERKEIFPT IPRLAPPASK INFQRSPSYI ELFQGMRVVL DKHDAHYNWK RLASQVSLSE 1260
GLKVNTEEDA AIINKSQDDA KAERMTQISE VIEYEMQQPI PTYLPKAHLD DSGIEKSDDK 1320
FFEIEEELKE ELKGSKTGNE DVGNNNPSNS IPKIEKPPAF KVIRTSPVRI IGRTFEDTRK 1380
YENGSPSDIS FTYDTHNNDE PDKRLMELKF PSQDEIPDDR FYTPAEEPTA EFPVEELPNT 1440
PRSINVTTSN NKSTDDKLSS GNIDQKPTEL LDDLEFSSFN IAFGNTSMST DNMKISSDLS 1500
SNKTVLGNAQ KVQESPSGPL IYVLPQSSTK HEKEGFLRKK QKDEPIWVSP SKIDFADLSR 1560
RTKALTPERN TVPLKNNDSR KYKYTGEGSI GNMTNMLLTK DASYAYLKDF VALSDDEDED 1620
GKQNCAVGGP EKLKFY 1636 
Gene Ontology
 GO:0000142; C:cellular bud neck contractile ring; IDA:SGD.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0007120; P:axial cellular bud site selection; IMP:SGD.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro. 
Interpro
 IPR000219; DH-domain.
 IPR021895; DUF3507. 
Pfam
 PF12015; DUF3507 
SMART
 SM00325; RhoGEF 
PROSITE
  
PRINTS