UniProt Accession

 GSTP1_MOUSE; P19157; Q8BNY4 

Genbank Protein ID

 X53451; X76143; U15654; D30687; BC002048; BC061109; AK079144 

Genbank Nucleotide ID

 CAA37529.1; AAA64837.1; BAA06349.1; AAH02048.1; AAH61109.1; BAC37560.1 

Protein Name

 Glutathione S-transferase P 1 

Protein Synonyms/Alias

 Gst P1; GST YF-YF; GST class-pi; GST-piB; Preadipocyte growth factor 

Gene Name

 Gstp1 

Gene Synonyms/Alias

 Gstpib 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
82	RSLGLYGKNQREAAQ	acetylation	[1, 2, 3]
82	RSLGLYGKNQREAAQ	succinylation	[2]
82	RSLGLYGKNQREAAQ	ubiquitination	[4]
103	GVEDLRGKYVTLIYT	acetylation	[1, 2, 3, 5, 6, 7]
103	GVEDLRGKYVTLIYT	succinylation	[2]
103	GVEDLRGKYVTLIYT	ubiquitination	[4]
116	YTNYENGKNDYVKAL	acetylation	[2, 6, 7]
116	YTNYENGKNDYVKAL	succinylation	[2]
116	YTNYENGKNDYVKAL	ubiquitination	[4]
121	NGKNDYVKALPGHLK	acetylation	[1, 2, 6, 7]
121	NGKNDYVKALPGHLK	succinylation	[2]
121	NGKNDYVKALPGHLK	ubiquitination	[4]
128	KALPGHLKPFETLLS	acetylation	[1, 2, 3, 5, 6, 7, 8, 9]
128	KALPGHLKPFETLLS	succinylation	[2]
128	KALPGHLKPFETLLS	ubiquitination	[4]
191	LSARPKIKAFLSSPE	acetylation	[2, 6]
191	LSARPKIKAFLSSPE	succinylation	[2]
191	LSARPKIKAFLSSPE	ubiquitination	[4]
209	RPINGNGKQ******	acetylation	[10]
209	RPINGNGKQ******	ubiquitination	[4]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [6] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [7] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [10] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Can metabolize 1-chloro-2,4-dinitrobenzene. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration (By similarity). 

Sequence Annotation

 DOMAIN 2 81 GST N-terminal.
 DOMAIN 83 204 GST C-terminal.
 REGION 52 53 Glutathione binding.
 REGION 65 66 Glutathione binding.
 BINDING 8 8 Glutathione.
 BINDING 14 14 Glutathione.
 BINDING 39 39 Glutathione.
 BINDING 45 45 Glutathione.
 MOD_RES 4 4 Phosphotyrosine; by EGFR (By similarity).
 MOD_RES 109 109 Phosphotyrosine.
 MOD_RES 128 128 N6-acetyllysine (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Mitochondrion; Nucleus; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 210 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; ISS:BHF-UCL.
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:BHF-UCL.
 GO:0005886; C:plasma membrane; ISS:BHF-UCL.
 GO:0043234; C:protein complex; IDA:BHF-UCL.
 GO:0008144; F:drug binding; ISS:BHF-UCL.
 GO:0004364; F:glutathione transferase activity; IDA:MGI.
 GO:0019207; F:kinase regulator activity; IMP:BHF-UCL.
 GO:0071460; P:cellular response to cell-matrix adhesion; ISS:BHF-UCL.
 GO:0071364; P:cellular response to epidermal growth factor stimulus; ISS:BHF-UCL.
 GO:0071385; P:cellular response to glucocorticoid stimulus; ISS:BHF-UCL.
 GO:0032869; P:cellular response to insulin stimulus; ISS:BHF-UCL.
 GO:0071222; P:cellular response to lipopolysaccharide; IDA:BHF-UCL.
 GO:0035726; P:common myeloid progenitor cell proliferation; IMP:BHF-UCL.
 GO:0006749; P:glutathione metabolic process; IDA:MGI.
 GO:0002674; P:negative regulation of acute inflammatory response; NAS:BHF-UCL.
 GO:0043066; P:negative regulation of apoptotic process; ISS:BHF-UCL.
 GO:0070373; P:negative regulation of ERK1 and ERK2 cascade; IDA:BHF-UCL.
 GO:0048147; P:negative regulation of fibroblast proliferation; IMP:BHF-UCL.
 GO:0043124; P:negative regulation of I-kappaB kinase/NF-kappaB cascade; IDA:BHF-UCL.
 GO:0032691; P:negative regulation of interleukin-1 beta production; IDA:BHF-UCL.
 GO:0043508; P:negative regulation of JUN kinase activity; IDA:BHF-UCL.
 GO:0070664; P:negative regulation of leukocyte proliferation; IMP:BHF-UCL.
 GO:0071638; P:negative regulation of monocyte chemotactic protein-1 production; IDA:BHF-UCL.
 GO:0060547; P:negative regulation of necrotic cell death; IDA:BHF-UCL.
 GO:2000429; P:negative regulation of neutrophil aggregation; NAS:BHF-UCL.
 GO:0051771; P:negative regulation of nitric-oxide synthase biosynthetic process; IDA:BHF-UCL.
 GO:2000469; P:negative regulation of peroxidase activity; IDA:BHF-UCL.
 GO:0032873; P:negative regulation of stress-activated MAPK cascade; IDA:BHF-UCL.
 GO:0032720; P:negative regulation of tumor necrosis factor production; IDA:BHF-UCL.
 GO:0014003; P:oligodendrocyte development; ISS:BHF-UCL.
 GO:0031100; P:organ regeneration; ISS:BHF-UCL.
 GO:0032930; P:positive regulation of superoxide anion generation; IMP:BHF-UCL.
 GO:0043200; P:response to amino acid stimulus; ISS:BHF-UCL.
 GO:0032355; P:response to estradiol stimulus; ISS:BHF-UCL.
 GO:0045471; P:response to ethanol; ISS:BHF-UCL.
 GO:0033591; P:response to L-ascorbic acid; ISS:BHF-UCL.
 GO:0000302; P:response to reactive oxygen species; IDA:BHF-UCL.
 GO:0009636; P:response to toxic substance; ISS:BHF-UCL.
 GO:0006805; P:xenobiotic metabolic process; ISS:UniProtKB. 

Interpro

 IPR010987; Glutathione-S-Trfase_C-like.
 IPR004045; Glutathione_S-Trfase_N.
 IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
 IPR004046; GST_C.
 IPR003082; GST_pi.
 IPR012336; Thioredoxin-like_fold. 

Pfam

 PF00043; GST_C
 PF02798; GST_N 

SMART

  

PROSITE

 PS50405; GST_CTER
 PS50404; GST_NTER 

PRINTS

 PR01268; GSTRNSFRASEP.