CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016213
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Telomerase-binding protein EST1A 
Protein Synonyms/Alias
 EST1-like protein A; Ever shorter telomeres 1A; Smg-6 homolog; Telomerase subunit EST1A; hSmg5/7a 
Gene Name
 SMG6 
Gene Synonyms/Alias
 C17orf31; EST1A; KIAA0732 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
441LLFGSGSKGSRSWGRubiquitination[1]
814MSLFEETKRKAEQMEubiquitination[2]
995CLLKESAKAQLSSPEubiquitination[3]
1152LLAFKGGKYVSVAPVubiquitination[2]
1225EQQRRQEKIQAVLEDubiquitination[2]
1309VVQEKARKSIEFLEQubiquitination[2]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. May have a general role in telomere regulation. Promotes in vitro the ability of TERT to elongate telomeres. Overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at the fusion points) and did not perturb TRF2 telomeric localization. Binds to the single-stranded 5'- (GTGTGG)(4)GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER). 
Sequence Annotation
 DOMAIN 1246 1397 PINc.
 REGION 114 503 Interaction with telomeric DNA.
 METAL 1251 1251 Manganese; catalytic (Probable).
 METAL 1353 1353 Manganese; catalytic (Probable).
 METAL 1392 1392 Manganese; catalytic (Probable).
 MOD_RES 479 479 Phosphothreonine.  
Keyword
 3D-structure; Alternative splicing; Chromosome; Coiled coil; Complete proteome; Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Manganese; Metal-binding; Nonsense-mediated mRNA decay; Nuclease; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Telomere. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1419 AA 
Protein Sequence
MAEGLERVRI SASELRGILA TLAPQAGSRE NMKELKEARP RKDNRRPDLE IYKPGLSRLR 60
NKPKIKEPPG SEEFKDEIVN DRDCSAVENG TQPVKDVCKE LNNQEQNGPI DPENNRGQES 120
FPRTAGQEDR SLKIIKRTKK PDLQIYQPGR RLQTVSKESA SRVEEEEVLN QVEQLRVEED 180
ECRGNVAKEE VANKPDRAEI EKSPGGGRVG AAKGEKGKRM GKGEGVRETH DDPARGRPGS 240
AKRYSRSDKR RNRYRTRSTS SAGSNNSAEG AGLTDNGCRR RRQDRTKERP RLKKQVSVSS 300
TDSLDEDRID EPDGLGPRRS SERKRHLERN WSGRGEGEQK NSAKEYRGTL RVTFDAEAMN 360
KESPMVRSAR DDMDRGKPDK GLSSGGKGSE KQESKNPKQE LRGRGRGILI LPAHTTLSVN 420
SAGSPESAPL GPRLLFGSGS KGSRSWGRGG TTRRLWDPNN PDQKPALKTQ TPQLHFLDTD 480
DEVSPTSWGD SRQAQASYYK FQNSDNPYYY PRTPGPASQY PYTGYNPLQY PVGPTNGVYP 540
GPYYPGYPTP SGQYVCSPLP TSTMSPEEVE QHMRNLQQQE LHRLLRVADN QELQLSNLLS 600
RDRISPEGLE KMAQLRAELL QLYERCILLD IEFSDNQNVD QILWKNAFYQ VIEKFRQLVK 660
DPNVENPEQI RNRLLELLDE GSDFFDSLLQ KLQVTYKFKL EDYMDGLAIR SKPLRKTVKY 720
ALISAQRCMI CQGDIARYRE QASDTANYGK ARSWYLKAQH IAPKNGRPYN QLALLAVYTR 780
RKLDAVYYYM RSLAASNPIL TAKESLMSLF EETKRKAEQM EKKQHEEFDL SPDQWRKGKK 840
STFRHVGDDT TRLEIWIHPS HPRSSQGTES GKDSEQENGL GSLSPSDLNK RFILSFLHAH 900
GKLFTRIGME TFPAVAEKVL KEFQVLLQHS PSPIGSTRML QLMTINMFAV HNSQLKDCFS 960
EECRSVIQEQ AAALGLAMFS LLVRRCTCLL KESAKAQLSS PEDQDDQDDI KVSSFVPDLK 1020
ELLPSVKVWS DWMLGYPDTW NPPPTSLDLP SHVAVDVWST LADFCNILTA VNQSEVPLYK 1080
DPDDDLTLLI LEEDRLLSGF VPLLAAPQDP CYVEKTSDKV IAADCKRVTV LKYFLEALCG 1140
QEEPLLAFKG GKYVSVAPVP DTMGKEMGSQ EGTRLEDEEE DVVIEDFEED SEAEGSGGED 1200
DIRELRAKKL ALARKIAEQQ RRQEKIQAVL EDHSQMRQME LEIRPLFLVP DTNGFIDHLA 1260
SLARLLESRK YILVVPLIVI NELDGLAKGQ ETDHRAGGYA RVVQEKARKS IEFLEQRFES 1320
RDSCLRALTS RGNELESIAF RSEDITGQLG NNDDLILSCC LHYCKDKAKD FMPASKEEPI 1380
RLLREVVLLT DDRNLRVKAL TRNVPVRDIP AFLTWAQVG 1419 
Gene Ontology
 GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005697; C:telomerase holoenzyme complex; TAS:HGNC.
 GO:0004521; F:endoribonuclease activity; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0042162; F:telomeric DNA binding; IDA:HGNC.
 GO:0010467; P:gene expression; TAS:Reactome.
 GO:0006406; P:mRNA export from nucleus; TAS:HGNC.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IMP:UniProtKB.
 GO:0035303; P:regulation of dephosphorylation; TAS:HGNC.
 GO:0000723; P:telomere maintenance; IMP:HGNC. 
Interpro
 IPR018834; DNA/RNA-bd_Est1-type.
 IPR019458; EST1.
 IPR002716; PIN_dom.
 IPR006596; PINc_nuc-bd.
 IPR011990; TPR-like_helical. 
Pfam
 PF10374; EST1
 PF10373; EST1_DNA_bind
 PF13638; PIN_4 
SMART
 SM00670; PINc 
PROSITE
  
PRINTS