CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023082
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-box/LRR-repeat protein 5 
Protein Synonyms/Alias
 F-box and leucine-rich repeat protein 5; F-box protein FBL4/FBL5; p45SKP2-like protein 
Gene Name
 FBXL5 
Gene Synonyms/Alias
 FBL4; FBL5; FLR1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
94EMLSLFEKGLKNVKNubiquitination[1]
100EKGLKNVKNEYEQLNubiquitination[1]
162IAQHCSQKDTAELLRubiquitination[2]
240MKWSQLTKTGSLWKHubiquitination[2]
276EPDDEWVKNRKDESRubiquitination[1]
403ITDVALEKISRALGIubiquitination[1, 2]
420SHQSGFLKTSTSKITubiquitination[2]
425FLKTSTSKITSTAWKubiquitination[2]
434TSTAWKNKDITMQSTubiquitination[2]
591LIYFGSEKSDQETGRubiquitination[1, 2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued. 
Sequence Annotation
 DOMAIN 202 248 F-box.
 REPEAT 340 364 LRR 1.
 REPEAT 365 392 LRR 2.
 REPEAT 393 418 LRR 3.
 REPEAT 479 508 LRR 4.
 REPEAT 576 607 LRR 5.
 REPEAT 608 635 LRR 6.
 REPEAT 636 661 LRR 7.
 REGION 1 159 Hemerythrin-like.
 METAL 15 15 Iron 1.
 METAL 57 57 Iron 1.
 METAL 58 58 Iron 1.
 METAL 61 61 Iron 1.
 METAL 61 61 Iron 2.
 METAL 80 80 Iron 2.
 METAL 126 126 Iron 2.
 METAL 130 130 Iron 1.
 METAL 130 130 Iron 2.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Iron; Leucine-rich repeat; Metal-binding; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 691 AA 
Protein Sequence
MAPFPEEVDV FTAPHWRMKQ LVGLYCDKLS KTNFSNNNDF RALLQSLYAT FKEFKMHEQI 60
ENEYIIGLLQ QRSQTIYNVH SDNKLSEMLS LFEKGLKNVK NEYEQLNYAK QLKERLEAFT 120
RDFLPHMKEE EEVFQPMLME YFTYEELKDI KKKVIAQHCS QKDTAELLRG LSLWNHAEER 180
QKFFKYSVDE KSDKEAEVSE HSTGITHLPP EVMLSIFSYL NPQELCRCSQ VSMKWSQLTK 240
TGSLWKHLYP VHWARGDWYS GPATELDTEP DDEWVKNRKD ESRAFHEWDE DADIDESEES 300
AEESIAISIA QMEKRLLHGL IHNVLPYVGT SVKTLVLAYS SAVSSKMVRQ ILELCPNLEH 360
LDLTQTDISD SAFDSWSWLG CCQSLRHLDL SGCEKITDVA LEKISRALGI LTSHQSGFLK 420
TSTSKITSTA WKNKDITMQS TKQYACLHDL TNKGIGEEID NEHPWTKPVS SENFTSPYVW 480
MLDAEDLADI EDTVEWRHRN VESLCVMETA SNFSCSTSGC FSKDIVGLRT SVCWQQHCAS 540
PAFAYCGHSF CCTGTALRTM SSLPESSAMC RKAARTRLPR GKDLIYFGSE KSDQETGRVL 600
LFLSLSGCYQ ITDHGLRVLT LGGGLPYLEH LNLSGCLTIT GAGLQDLVSA CPSLNDEYFY 660
YCDNINGPHA DTASGCQNLQ CGFRACCRSG E 691 
Gene Ontology
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0019005; C:SCF ubiquitin ligase complex; IDA:UniProtKB.
 GO:0005506; F:iron ion binding; IDA:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; NAS:UniProtKB.
 GO:0055072; P:iron ion homeostasis; IMP:UniProtKB.
 GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB. 
Interpro
 IPR001810; F-box_dom_cyclin-like.
 IPR012312; Haemerythrin/HHE_cat-bd_motif.
 IPR006553; Leu-rich_rpt_Cys-con_subtyp. 
Pfam
 PF01814; Hemerythrin 
SMART
 SM00256; FBOX
 SM00367; LRR_CC 
PROSITE
 PS50181; FBOX 
PRINTS