UniProt Accession

 EPM2A_HUMAN; O95278; B3KMU5; B4DRZ2; O95483; Q5T3F5; Q6IS15; Q8IU96; Q8IX24; Q8IX25; Q9BS66; Q9UEN2 

Genbank Protein ID

 AF084535; AF284580; AF454491; AF454492; AF454493; AF454494; AK022721; AK299497; AL023806; AL365193; AL365193; AL023806; CH471051; BC005286; BC070047; AJ130763; AJ130764 

Genbank Nucleotide ID

 AAC83347.2; AAG18377.1; AAO15523.1; AAO15524.1; AAO15525.1; AAO15526.1; BAG51107.1; BAG61454.1; CAI21419.1; CAI21419.1; CAI21675.1; CAI21675.1; EAW47844.1; AAH05286.1; AAH70047.1; CAA10199.1; CAA10200.1 

Protein Name

 Laforin 

Protein Synonyms/Alias

 Lafora PTPase; LAFPTPase 

Gene Name

 EPM2A 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
320	AQEDFFQKFGKVRSS	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Dual specificity protein phosphatase. May be involved in the control of glycogen metabolism, particularly in monitoring for and preventing the formation of poorly branched glycogen molecules (polyglucosans). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin. Forms a complex with NHLRC1/malin and HSP70 and this complex suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Isoform 2, an inactive phosphatase, could function as a dominant-negative regulator for the phosphatase activity of isoform 1. 

Sequence Annotation

 DOMAIN 1 124 CBM20.
 DOMAIN 243 311 Tyrosine-protein phosphatase.
 MOD_RES 25 25 Phosphoserine; by AMPK.  

Keyword

 Alternative initiation; Alternative splicing; Carbohydrate metabolism; Cell membrane; Complete proteome; Cytoplasm; Disease mutation; Endoplasmic reticulum; Epilepsy; Glycogen metabolism; Hydrolase; Membrane; Nucleus; Phosphoprotein; Polymorphism; Protein phosphatase; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 331 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005844; C:polysome; IDA:UniProtKB.
 GO:0004722; F:protein serine/threonine phosphatase activity; IDA:UniProtKB.
 GO:0004725; F:protein tyrosine phosphatase activity; IDA:UniProtKB.
 GO:0008138; F:protein tyrosine/serine/threonine phosphatase activity; IEA:InterPro.
 GO:2001070; F:starch binding; IEA:InterPro.
 GO:0007610; P:behavior; IEA:Compara.
 GO:0005977; P:glycogen metabolic process; NAS:UniProtKB.
 GO:0007399; P:nervous system development; IEA:Compara. 

Interpro

 IPR013784; Carb-bd-like_fold.
 IPR002044; CBM_fam20.
 IPR000340; Dual-sp_phosphatase_cat-dom.
 IPR020422; Dual-sp_phosphatase_subgr_cat.
 IPR024950; DUSP.
 IPR013783; Ig-like_fold.
 IPR000387; Tyr/Dual-sp_Pase.
 IPR016130; Tyr_Pase_AS. 

Pfam

 PF00686; CBM_20
 PF00782; DSPc 

SMART

 SM01065; CBM_2 

PROSITE

 PS51166; CBM20
 PS00383; TYR_PHOSPHATASE_1
 PS50056; TYR_PHOSPHATASE_2
 PS50054; TYR_PHOSPHATASE_DUAL 

PRINTS