CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023294
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA-binding protein SATB2 
Protein Synonyms/Alias
 Special AT-rich sequence-binding protein 2 
Gene Name
 SATB2 
Gene Synonyms/Alias
 KIAA1034 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
184NALKELLKEMNQSTLubiquitination[1, 2]
233YKKYKKIKVERVEREsumoylation[3]
350PPIPRAVKPEPTNSSsumoylation[3, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] SUMO modification of a novel MAR-binding protein, SATB2, modulates immunoglobulin mu gene expression.
 Dobreva G, Dambacher J, Grosschedl R.
 Genes Dev. 2003 Dec 15;17(24):3048-61. [PMID: 14701874]
 [4] Targeted identification of SUMOylation sites in human proteins using affinity enrichment and paralog-specific reporter ions.
 Lamoliatte F, Bonneil E, Durette C, Caron-Lizotte O, Wildemann D, Zerweck J, Wenschuh H, Thibault P.
 Mol Cell Proteomics. 2013 Jun 7;. [PMID: 23750026] 
Functional Description
 Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper- layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. 
Sequence Annotation
 DNA_BIND 350 437 CUT 1.
 DNA_BIND 473 560 CUT 2.
 DNA_BIND 615 674 Homeobox.
 CROSSLNK 233 233 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 350 350 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Chromatin regulator; Chromosomal rearrangement; Complete proteome; Developmental protein; Disease mutation; DNA-binding; Homeobox; Isopeptide bond; Mental retardation; Nucleus; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 733 AA 
Protein Sequence
MERRSESPCL RDSPDRRSGS PDVKGPPPVK VARLEQNGSP MGARGRPNGA VAKAVGGLMI 60
PVFCVVEQLD GSLEYDNREE HAEFVLVRKD VLFSQLVETA LLALGYSHSS AAQAQGIIKL 120
GRWNPLPLSY VTDAPDATVA DMLQDVYHVV TLKIQLQSCS KLEDLPAEQW NHATVRNALK 180
ELLKEMNQST LAKECPLSQS MISSIVNSTY YANVSATKCQ EFGRWYKKYK KIKVERVERE 240
NLSDYCVLGQ RPMHLPNMNQ LASLGKTNEQ SPHSQIHHST PIRNQVPALQ PIMSPGLLSP 300
QLSPQLVRQQ IAMAHLINQQ IAVSRLLAHQ HPQAINQQFL NHPPIPRAVK PEPTNSSVEV 360
SPDIYQQVRD ELKRASVSQA VFARVAFNRT QGLLSEILRK EEDPRTASQS LLVNLRAMQN 420
FLNLPEVERD RIYQDERERS MNPNVSMVSS ASSSPSSSRT PQAKTSTPTT DLPIKVDGAN 480
INITAAIYDE IQQEMKRAKV SQALFAKVAA NKSQGWLCEL LRWKENPSPE NRTLWENLCT 540
IRRFLNLPQH ERDVIYEEES RHHHSERMQH VVQLPPEPVQ VLHRQQSQPA KESSPPREEA 600
PPPPPPTEDS CAKKPRSRTK ISLEALGILQ SFIHDVGLYP DQEAIHTLSA QLDLPKHTII 660
KFFQNQRYHV KHHGKLKEHL GSAVDVAEYK DEELLTESEE NDSEEGSEEM YKVEAEEENA 720
DKSKAAPAEI DQR 733 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0000118; C:histone deacetylase complex; IEA:Compara.
 GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0051216; P:cartilage development; IEA:Compara.
 GO:0071310; P:cellular response to organic substance; IEA:Compara.
 GO:0006338; P:chromatin remodeling; IEA:Compara.
 GO:0009880; P:embryonic pattern specification; IEA:Compara.
 GO:0048704; P:embryonic skeletal system morphogenesis; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0001764; P:neuron migration; IEA:Compara.
 GO:0002076; P:osteoblast development; IEA:Compara.
 GO:0060021; P:palate development; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR003350; Hmoeo_CUT.
 IPR001356; Homeodomain.
 IPR009057; Homeodomain-like.
 IPR010982; Lambda_DNA-bd_dom. 
Pfam
 PF02376; CUT
 PF00046; Homeobox 
SMART
 SM00389; HOX 
PROSITE
 PS51042; CUT
 PS00027; HOMEOBOX_1
 PS50071; HOMEOBOX_2 
PRINTS