CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037128
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like 
Protein Synonyms/Alias
  
Gene Name
 DUS2L 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
111KILSTLVKGTRRPVTubiquitination[1, 2]
120TRRPVTCKIRILPSLubiquitination[1, 3]
135EDTLSLVKRIERTGIubiquitination[1, 2, 4, 5]
231PLEEVMQKYIRYAVQubiquitination[1]
302QQARLSAKTSEQTGEubiquitination[1, 2, 3, 5]
319EDTSGVIKMAVKFDRubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 458 AA 
Protein Sequence
MILNSLSLCY HNKLILAPMV RVGTLPMRLL ALDYGADIVY CEELIDLKMI QCKRVVNEVL 60
STVDFVAPDD RVVFRTCERE QNRVVFQMGG MGAALLSDPD KIEKILSTLV KGTRRPVTCK 120
IRILPSLEDT LSLVKRIERT GIAAIAVHGR KREERPQHPV SCEVIKAIAD TLSIPVIANG 180
GSHDHIQQYS DIEDFRQATA ASSVMVARAA MWNPSIFLKE GLRPLEEVMQ KYIRYAVQYD 240
NHYTNTKYCL CQMLREQLES PQGRLLHAAQ SSREICEAFG LGAFYEETTQ ELDAQQARLS 300
AKTSEQTGEP AEDTSGVIKM AVKFDRRAYP AQITPKMCLL EWCRREKLAQ PVYETVQRPL 360
DRLFSSIVTV AEQKYQSTLW DKSKKLAEQA AAIVCLRSQG LPEGRLGEES PSLHKRKREA 420
PDQDPGGPRA QELAQPGDLC KKPFVALGSG EESPLEGW 458 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0017150; F:tRNA dihydrouridine synthase activity; IEA:InterPro.
 GO:0002943; P:tRNA dihydrouridine synthesis; IEA:GOC. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR001159; Ds-RNA-bd.
 IPR014720; dsRNA-bd-like_dom.
 IPR001269; tRNA_hU_synthase. 
Pfam
 PF00035; dsrm
 PF01207; Dus 
SMART
 SM00358; DSRM 
PROSITE
  
PRINTS