UniProt Accession

 EDE1_YEAST; P34216; D6VPV1 

Genbank Protein ID

 Z35808; X78214; Z23261; BK006936 

Genbank Nucleotide ID

 CAA84867.1; CAA55048.1; CAA80797.1; DAA07071.1 

Protein Name

 EH domain-containing and endocytosis protein 1 

Protein Synonyms/Alias

 Bud site selection protein 15 

Gene Name

 EDE1 

Gene Synonyms/Alias

 BUD15; YBL047C; YBL0501; YBL0520 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
157	AQTVAGDKAKDIFLK	ubiquitination	[1]
498	NFGQSIIKEEPEEQE	ubiquitination	[1]
674	AQVLQVNKENETLAQ	ubiquitination	[2]
780	KISVYLTKQKELNDY	ubiquitination	[1]
782	SVYLTKQKELNDYQK	ubiquitination	[1]
808	KYQDLSNKDTDLTDR	acetylation	[3]
892	GQLPEDAKDIIAKSA	ubiquitination	[1]
897	DAKDIIAKSASNTDT	ubiquitination	[1]
907	SNTDTTTKEATSRGN	ubiquitination	[1]
1083	IEDGSTTKRANSNED	ubiquitination	[1]
1102	SSIQESPKISAQPKA	ubiquitination	[1]
1108	PKISAQPKAKTINEE	ubiquitination	[1]
1149	EIPSATVKTLQTPYN	ubiquitination	[1]
1304	NSKAEPTKVATPSIP	ubiquitination	[1]
1318	PQQPIPLKNDPIVDA	ubiquitination	[1]
1329	IVDASLSKGPIVNRG	ubiquitination	[1, 2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Functions at the internalization step of the clathrin- mediated endocytosis (CME) as an early-acting scaffold protein. Requires clathrin adapter proteins, ENT1/2 and YAP1801/2, for normal spatiotemporal dynamics and viability. Binds to biological membranes in a ubiquitin-dependent manner. 

Sequence Annotation

 DOMAIN 14 113 EH 1.
 DOMAIN 135 227 EH 2.
 DOMAIN 277 366 EH 3.
 DOMAIN 1338 1380 UBA.
 REGION 1217 1381 Able to bind biological membranes.
 MOD_RES 238 238 Phosphothreonine.
 MOD_RES 241 241 Phosphoserine.
 MOD_RES 244 244 Phosphoserine.
 MOD_RES 245 245 Phosphothreonine.
 MOD_RES 248 248 Phosphoserine.
 MOD_RES 249 249 Phosphoserine.
 MOD_RES 251 251 Phosphothreonine.
 MOD_RES 265 265 Phosphoserine.
 MOD_RES 419 419 Phosphoserine.
 MOD_RES 450 450 Phosphothreonine.
 MOD_RES 477 477 Phosphothreonine.
 MOD_RES 487 487 Phosphothreonine.
 MOD_RES 495 495 Phosphoserine.
 MOD_RES 848 848 Phosphoserine.
 MOD_RES 931 931 Phosphoserine.
 MOD_RES 950 950 Phosphoserine.
 MOD_RES 964 964 Phosphoserine.
 MOD_RES 1008 1008 Phosphoserine.
 MOD_RES 1012 1012 Phosphoserine.
 MOD_RES 1020 1020 Phosphoserine.
 MOD_RES 1046 1046 Phosphothreonine.
 MOD_RES 1069 1069 Phosphoserine.
 MOD_RES 1087 1087 Phosphoserine.
 MOD_RES 1093 1093 Phosphoserine.
 MOD_RES 1095 1095 Phosphoserine.
 MOD_RES 1096 1096 Phosphoserine.
 MOD_RES 1100 1100 Phosphoserine.
 MOD_RES 1111 1111 Phosphothreonine.
 MOD_RES 1181 1181 Phosphoserine.
 MOD_RES 1187 1187 Phosphoserine.
 MOD_RES 1307 1307 Phosphothreonine.
 MOD_RES 1343 1343 Phosphoserine.  

Keyword

 3D-structure; Coiled coil; Complete proteome; Cytoplasm; Endocytosis; Phosphoprotein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1381 AA 

Protein Sequence

Gene Ontology

 GO:0030479; C:actin cortical patch; IDA:SGD.
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005934; C:cellular bud tip; IDA:SGD.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0043130; F:ubiquitin binding; IDA:SGD.
 GO:0006897; P:endocytosis; IMP:SGD.
 GO:0030968; P:endoplasmic reticulum unfolded protein response; IMP:SGD. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology.
 IPR009060; UBA-like.
 IPR000449; UBA/transl_elong_EF1B_N.
 IPR015940; UBA/transl_elong_EF1B_N_euk. 

Pfam

 PF00627; UBA 

SMART

 SM00054; EFh
 SM00027; EH
 SM00165; UBA 

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH
 PS50030; UBA 

PRINTS