CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022735
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Peroxisomal acyl-coenzyme A oxidase 1 
Protein Synonyms/Alias
 AOX; Palmitoyl-CoA oxidase 
Gene Name
 Acox1 
Gene Synonyms/Alias
 Acox; Paox 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
65QRYEVAVKKSATMVKacetylation[1, 2, 3]
65QRYEVAVKKSATMVKubiquitination[4]
72KKSATMVKKMREFGIacetylation[3]
89PEEIMWFKKLHMVNFacetylation[5]
89PEEIMWFKKLHMVNFsuccinylation[5]
89PEEIMWFKKLHMVNFubiquitination[4]
90EEIMWFKKLHMVNFVacetylation[5]
90EEIMWFKKLHMVNFVsuccinylation[5]
90EEIMWFKKLHMVNFVubiquitination[4]
159TTATYDPKTQEFILNubiquitination[4]
174SPTVTSIKWWPGGLGubiquitination[4]
216IREIGTHKPLPGITVacetylation[1]
216IREIGTHKPLPGITVubiquitination[4]
229TVGDIGPKFGYEEMDubiquitination[4]
241EMDNGYLKMDNYRIPubiquitination[4]
255PRENMLMKYAQVKPDacetylation[1]
260LMKYAQVKPDGTYVKacetylation[1]
260LMKYAQVKPDGTYVKubiquitination[4]
267KPDGTYVKPLSNKLTacetylation[1, 6]
267KPDGTYVKPLSNKLTubiquitination[4]
272YVKPLSNKLTYGTMVacetylation[1]
272YVKPLSNKLTYGTMVubiquitination[4]
313VRRQSEIKRSEPEPQacetylation[1]
349HFLGRYIKETYMRINacetylation[1]
349HFLGRYIKETYMRINubiquitination[4]
437QTARFLMKIYDQVQSacetylation[1]
437QTARFLMKIYDQVQSubiquitination[4]
446YDQVQSGKLVGGMVSubiquitination[4]
488DSLTEAYKLRAARLVubiquitination[4]
500RLVEIAAKNLQAQVSubiquitination[4]
512QVSHRKSKEVAWNLTacetylation[1]
512QVSHRKSKEVAWNLTubiquitination[4]
542TVKVFADKLPKIQDRacetylation[1]
542TVKVFADKLPKIQDRubiquitination[4]
545VFADKLPKIQDRAVQacetylation[1]
637ENLFEWAKKSPLNKTacetylation[1]
643AKKSPLNKTEVHESYacetylation[1, 6]
643AKKSPLNKTEVHESYubiquitination[4]
652EVHESYYKHLKPLQSubiquitination[4]
655ESYYKHLKPLQSKL*acetylation[1]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [6] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647] 
Functional Description
 Catalyzes the desaturation of acyl-CoAs to 2-trans- enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs (By similarity). 
Sequence Annotation
 MOTIF 659 661 Microbody targeting signal.
 ACT_SITE 421 421 Proton acceptor (By similarity).
 BINDING 139 139 FAD (By similarity).
 BINDING 178 178 FAD; via amide nitrogen (By similarity).
 MOD_RES 26 26 Phosphoserine (By similarity).
 MOD_RES 255 255 N6-acetyllysine (By similarity).
 MOD_RES 267 267 N6-acetyllysine.
 MOD_RES 310 310 Phosphoserine.
 MOD_RES 437 437 N6-acetyllysine (By similarity).
 MOD_RES 500 500 N6-acetyllysine (By similarity).
 MOD_RES 643 643 N6-acetyllysine.
 MOD_RES 649 649 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; FAD; Fatty acid metabolism; Flavoprotein; Lipid metabolism; Oxidoreductase; Peroxisome; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 661 AA 
Protein Sequence
MNPDLRKERA AATFNPELIT HILDGSPENT RRRREIENLI LNDPDFQHED YNFLTRSQRY 60
EVAVKKSATM VKKMREFGIA DPEEIMWFKK LHMVNFVEPV GLNYSMFIPT LLNQGTTAQQ 120
EKWMHPSQEL QIIGTYAQTE MGHGTHLRGL ETTATYDPKT QEFILNSPTV TSIKWWPGGL 180
GKTSNHAIVL AQLITRGECY GLHAFVVPIR EIGTHKPLPG ITVGDIGPKF GYEEMDNGYL 240
KMDNYRIPRE NMLMKYAQVK PDGTYVKPLS NKLTYGTMVF VRSFLVGSAA QSLSKACTIA 300
IRYSAVRRQS EIKRSEPEPQ ILDFQTQQYK LFPLLATAYA FHFLGRYIKE TYMRINESIG 360
QGDLSELPEL HALTAGLKAF TTWTANAGIE ECRMACGGHG YSHSSGIPNI YVTFTPACTF 420
EGENTVMMLQ TARFLMKIYD QVQSGKLVGG MVSYLNDLPS QRIQPQQVAV WPTLVDINSL 480
DSLTEAYKLR AARLVEIAAK NLQAQVSHRK SKEVAWNLTS VDLVRASEAH CHYVTVKVFA 540
DKLPKIQDRA VQAVLRNLCL LYSLYGISQK GGDFLEGNII TGAQMSQVNS RILELLTVTR 600
PNAVALVDAF DFKDVTLGSV LGRYDGNVYE NLFEWAKKSP LNKTEVHESY YKHLKPLQSK 660
L 661 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005778; C:peroxisomal membrane; IDA:MGI.
 GO:0003995; F:acyl-CoA dehydrogenase activity; IEA:InterPro.
 GO:0003997; F:acyl-CoA oxidase activity; TAS:MGI.
 GO:0071949; F:FAD binding; IEA:Compara.
 GO:0005504; F:fatty acid binding; IEA:Compara.
 GO:0016401; F:palmitoyl-CoA oxidase activity; IEA:Compara.
 GO:0006635; P:fatty acid beta-oxidation; TAS:MGI.
 GO:0033540; P:fatty acid beta-oxidation using acyl-CoA oxidase; IEA:UniProtKB-UniPathway.
 GO:0006091; P:generation of precursor metabolites and energy; IEA:Compara.
 GO:0055088; P:lipid homeostasis; IEA:Compara.
 GO:0016559; P:peroxisome fission; IEA:Compara.
 GO:2000189; P:positive regulation of cholesterol homeostasis; IEA:Compara.
 GO:0006693; P:prostaglandin metabolic process; IEA:Compara.
 GO:0007283; P:spermatogenesis; IMP:MGI.
 GO:0000038; P:very long-chain fatty acid metabolic process; IEA:Compara. 
Interpro
 IPR006091; Acyl-CoA_Oxase/DH_cen-dom.
 IPR012258; Acyl-CoA_oxidase.
 IPR002655; Acyl-CoA_oxidase_C.
 IPR009075; AcylCo_DH/oxidase_C.
 IPR013786; AcylCoA_DH/ox_N.
 IPR009100; AcylCoA_DH/oxidase. 
Pfam
 PF01756; ACOX
 PF02770; Acyl-CoA_dh_M 
SMART
  
PROSITE
  
PRINTS