CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037655
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Cofilin-1 
Protein Synonyms/Alias
  
Gene Name
 CFL1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
51AVSDGVIKVFNDMKVacetylation[1]
51AVSDGVIKVFNDMKVubiquitination[2, 3, 4, 5, 6, 7]
57IKVFNDMKVRKSSTPacetylation[1, 7, 8]
57IKVFNDMKVRKSSTPubiquitination[3, 4, 5, 6, 7, 9, 10, 11]
60FNDMKVRKSSTPEEVubiquitination[6]
68SSTPEEVKKRKKAVLacetylation[7]
71PEEVKKRKKAVLFCLubiquitination[4]
72EEVKKRKKAVLFCLSubiquitination[5]
82LFCLSEDKKNIILEEacetylation[7, 8]
82LFCLSEDKKNIILEEubiquitination[6]
83FCLSEDKKNIILEEGubiquitination[9]
91NIILEEGKEILVGDVubiquitination[4, 9]
111DPYATFVKMLPDKDCacetylation[8]
111DPYATFVKMLPDKDCubiquitination[3, 5, 9, 10, 11, 12]
116FVKMLPDKDCRYALYacetylation[7]
116FVKMLPDKDCRYALYubiquitination[6, 7]
130YDATYETKESKKEDLacetylation[1, 7, 8]
130YDATYETKESKKEDLubiquitination[2, 4, 5, 6, 7, 9, 10, 11, 12]
133TYETKESKKEDLVFIubiquitination[4, 6, 7, 11]
134YETKESKKEDLVFIFubiquitination[9, 11]
150APESAPLKSKMIYASubiquitination[2, 3, 4, 6, 9, 10, 11, 12]
152ESAPLKSKMIYASSKubiquitination[3, 4, 6, 7, 9, 10, 11]
159KMIYASSKDAIKKKLacetylation[1, 7]
159KMIYASSKDAIKKKLubiquitination[4, 6, 7, 10, 11]
165SKDAIKKKLTGIKHEubiquitination[3]
170KKKLTGIKHELQANCacetylation[7, 8]
170KKKLTGIKHELQANCubiquitination[4, 6, 7, 10]
182ANCYEEVKDRCTLAEacetylation[1, 7]
182ANCYEEVKDRCTLAEubiquitination[4, 6, 7, 9, 10, 11, 12]
190DRCTLAEKLGGSAVIubiquitination[6]
202AVISLEGKPL*****ubiquitination[6, 7, 9, 11]
Reference
 [1] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [2] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [10] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [11] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [12] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
  
Keyword
 Actin-binding; Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 204 AA 
Protein Sequence
MVSPGHGHLR ERKELTPHST NPAAARIWDA TYSDGYVRPA SGVAVSDGVI KVFNDMKVRK 60
SSTPEEVKKR KKAVLFCLSE DKKNIILEEG KEILVGDVGQ TVDDPYATFV KMLPDKDCRY 120
ALYDATYETK ESKKEDLVFI FWAPESAPLK SKMIYASSKD AIKKKLTGIK HELQANCYEE 180
VKDRCTLAEK LGGSAVISLE GKPL 204 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IEA:InterPro.
 GO:0030042; P:actin filament depolymerization; IEA:InterPro. 
Interpro
 IPR002108; Actin-bd_cofilin/tropomyosin.
 IPR017904; ADF/Cofilin/Destrin.
 IPR027234; Cofilin_1/2. 
Pfam
 PF00241; Cofilin_ADF 
SMART
 SM00102; ADF 
PROSITE
 PS51263; ADF_H 
PRINTS
 PR00006; COFILIN.