CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015186
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Exportin-1 
Protein Synonyms/Alias
 Exp1; Chromosome region maintenance 1 protein homolog 
Gene Name
 Xpo1 
Gene Synonyms/Alias
 Crm1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
446EVVREFMKDTDSINLacetylation[1]
455TDSINLYKNMRETLVacetylation[2]
455TDSINLYKNMRETLVubiquitination[3]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap (By similarity). 
Sequence Annotation
 DOMAIN 46 112 Importin N-terminal.
 REPEAT 217 240 HEAT 1.
 REPEAT 241 277 HEAT 2.
 REPEAT 354 472 HEAT 3.
 REPEAT 515 553 HEAT 4.
 REPEAT 560 597 HEAT 5.
 REPEAT 602 639 HEAT 6.
 REPEAT 775 813 HEAT 7.
 REPEAT 885 916 HEAT 8.
 REPEAT 917 954 HEAT 9.
 REPEAT 1002 1039 HEAT 10.
 REGION 327 450 Necessary for interaction with Ran and
 REGION 411 481 Necessary for interaction with RANBP3 (By
 MOD_RES 391 391 Phosphoserine (By similarity).
 MOD_RES 446 446 N6-acetyllysine (By similarity).
 MOD_RES 448 448 Phosphothreonine (By similarity).
 MOD_RES 450 450 Phosphoserine (By similarity).
 MOD_RES 454 454 Phosphotyrosine (By similarity).
 MOD_RES 693 693 N6-acetyllysine (By similarity).  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; mRNA transport; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; RNA-binding; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1071 AA 
Protein Sequence
MPAIMTMLAD HAARQLLDFS QKLDINLLDN VVNCLYHGEG AQQRMAQEVL THLKEHPDAW 60
TRVDTILEFS QNMNTKYYGL QILENVIKTR WKILPRNQCE GIKKYVVGLI IKTSSDPTCV 120
EKEKVYIGKL NMILVQILKQ EWPKHWPTFI SDIVGASRTS ESLCQNNMVI LKLLSEEVFD 180
FSSGQITQVK AKHLKDSMCN EFSQIFQLCQ FVMENSQNAP LVHATLETLL RFLNWIPLGY 240
IFETKLISTL IYKFLNVPMF RNVSLKCLTE IAGVSVSQYE EQFETLFTLT MMQLKQMLPL 300
NTNIRLAYSN GKDDEQNFIQ NLSLFLCTFL KEHGQLLEKR LNLREALMEA LHYMLLVSEV 360
EETEIFKICL EYWNHLAAEL YRESPFSTSA SPLLSGSQHF DIPPRRQLYL TVLSKVRLLM 420
VSRMAKPEEV LVVENDQGEV VREFMKDTDS INLYKNMRET LVYLTHLDYV DTEIIMTKKL 480
QNQVNGTEWS WKNLNTLCWA IGSISGAMHE EDEKRFLVTV IKDLLGLCEQ KRGKDNKAII 540
ASNIMYIVGQ YPRFLRAHWK FLKTVVNKLF EFMHETHDGV QDMACDTFIK IAQKCRRHFV 600
QVQVGEVMPF IDEILNNINT IICDLQPQQV HTFYEAVGYM IGAQTDQTVQ EHLIEKYMLL 660
PNQVWDSIIQ QATKNVDILK DPETVKQLGS ILKTNVRACK AVGHPFVIQL GRIYLDMLNV 720
YKCLSENISA AIQANGEMVT KQPLIRSMRT VKRETLKLIS GWVSRSNDPQ MVAENFVPPL 780
LDAVLIDYQR NVPAAREPEV LSTMAIIVNK LGGHITAEIP QIFDAVFECT LNMINKDFEE 840
YPEHRTNFFL LLQAVNSHCF PAFLAIPPAQ FKLVLDSIIW AFKHTMRNVA DTGLQILFTL 900
LQNVAQEEAA AQSFYQTYFC DILQHIFSVV TDTSHTAGLT MHASILAYMF NLVEEGKIST 960
PLNPGNPVNN QMFIQDYVAN LLKSAFPHLQ DAQVKLFVTG LFSLNQDIPA FKEHLRDFLV 1020
QIKEFAGEDT SDLFLEERET ALRQAQEEKH KLQMSVPGIL NPHEIPEEMC D 1071 
Gene Ontology
 GO:0005642; C:annulate lamellae; IEA:Compara.
 GO:0015030; C:Cajal body; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0000776; C:kinetochore; ISS:UniProtKB.
 GO:0005643; C:nuclear pore; IEA:InterPro.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0030529; C:ribonucleoprotein complex; ISO:MGI.
 GO:0008565; F:protein transporter activity; IDA:MGI.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006611; P:protein export from nucleus; IDA:MGI.
 GO:0034504; P:protein localization to nucleus; IMP:MGI.
 GO:0010824; P:regulation of centrosome duplication; IMP:MGI.
 GO:0042176; P:regulation of protein catabolic process; IMP:MGI.
 GO:0046825; P:regulation of protein export from nucleus; IMP:MGI.
 GO:0042493; P:response to drug; IEA:Compara. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR014877; CRM1_C_dom.
 IPR013598; Exportin-1/Importin-b-like.
 IPR001494; Importin-beta_N. 
Pfam
 PF08767; CRM1_C
 PF03810; IBN_N
 PF08389; Xpo1 
SMART
 SM01102; CRM1_C
 SM00913; IBN_N 
PROSITE
 PS50077; HEAT_REPEAT
 PS50166; IMPORTIN_B_NT 
PRINTS